Linking DNA-binding proteins to their recognition sequences by using protein microarrays

被引:48
作者
Ho, Su-Wen
Jona, Ghil
Chen, Christina T. L.
Johnston, Mark
Snyder, Michael
机构
[1] Washington Univ, Sch Med, St Louis, MO 63108 USA
[2] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
关键词
proteomics; transcription factor; yeast;
D O I
10.1073/pnas.0509185103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Analyses of whole-genome sequences and experimental data sets have revealed a large number of DNA sequence motifs that are conserved in many species and may be functional. However, methods of sufficient scale to explore the roles of these elements are lacking. We describe the use of protein arrays to identify proteins that bind to DNA sequences of interest. A microarray of 282 known and potential yeast transcription factors was produced and probed with oligonucleotides of evolutionarily conserved sequences that are potentially functional. Transcription factors that bound to specific DNA sequences were identified. One previously uncharacterized DNA-binding protein, YjI103, was characterized in detail. We defined the binding site for this protein and identified a number of its target genes, many of which are involved in stress response and oxidative phosphorylation. Protein microarrays offer a high-throughput method for determining DNA-protein interactions.
引用
收藏
页码:9940 / 9945
页数:6
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