Formation and stability of complex membrane-mimetic monolayers on solid supports

被引:31
作者
Winger, TM
Ludovice, PJ
Chaikof, EL
机构
[1] Emory Univ, Sch Med, Lab Biomol Mat Res, Dept Surg, Atlanta, GA 30322 USA
[2] Georgia Inst Technol, Sch Chem Engn, Atlanta, GA 30322 USA
关键词
D O I
10.1021/la980990q
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Membrane-mimetic phospholipid films with or without cholesterol and varying concentrations of one or more synthetic lipopeptide conjugates were formed on an alkylsilane self-assembled monolayer on glass by a process of lipid vesicle fusion. Using a combination of radiochemical titration with I-125-labeled lipopeptides and atomic force microscopy imaging in an aqueous environment, we have characterized the kinetics of multicomponent monolayer formation and film stability as a function of temperature, lipid alkyl chain length, cholesterol content, and the concentration of solution phase bovine serum albumin. These data confirm that multicomponent membrane-mimetic monolayers can be produced in a predictable fashion by vesicle fusion to an alkylated glass substrate. The stability of these systems, however, is compromised when operating above the phase transition temperature of the lipid component, as well as in the presence of albumin.
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页码:3866 / 3874
页数:9
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