FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes

被引:82
作者
Golic, MM
Rong, YS
Petersen, RB
Lindquist, SL
Golic, KG
机构
[1] UNIV UTAH,DEPT BIOL,SALT LAKE CITY,UT 84112
[2] UNIV CHICAGO,HOWARD HUGHES MED INST,CHICAGO,IL 60637
[3] CASE WESTERN RESERVE UNIV,INST PATHOL,CLEVELAND,OH 44106
关键词
D O I
10.1093/nar/25.18.3665
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ability to place a series of gene constructs at a specific site in the genome opens new possibilities for the experimental examination of gene expression and chromosomal position effects. We report that the FLP-FRT site-specific recombination system of the yeast 2 mu plasmid can be used to integrate DNA at a chromosomal FRT target site in Drosophila. The technique we used was to first integrate an FRT-flanked gene by standard P element-mediated transformation, FLP was then used to excise the FRT flanked donor DNA and screen for FLP-mediated re-integration at an FRT target at a different chromosome location, Such events were recovered from up to 5% of the crosses used to screen for mobilization and are easily detectable by altered linkage of a white reporter gene or by the generation of a white(+) gene upon integration.
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页码:3665 / 3671
页数:7
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