Two proteins share immunological epitopes on the tumor-associated antigen 17-1A

被引:2
作者
Chen, YH [1 ]
Yu, TW [1 ]
Bai, Y [1 ]
Zhao, NM [1 ]
机构
[1] Tsinghua Univ, Sch Life Sci & Engn, Beijing 100084, Peoples R China
关键词
tumor-associated antigen; EpCAM; 17-1A; common immunological epitope;
D O I
10.1016/S0304-3835(99)00205-0
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The mouse monoclonal antibody (mAb) 17-1A which recognizes the tumor-associated antigen 17-1A (also called EGP-40 or EpCAM) was successfully used in adjuvant therapy for colorectal carcinoma. In the 17-1A antigen analysis, we isolated not only a protein of 33 kDa (P33) which was reported as the tumor associated antigen 17-1A, but also a protein of 65 kDa (P65) using affinity chromatography from cell lysates of HCT, and another protein of 50 kDa (P50) from lysates of human colorectal tumor tissues. The mAbs 17-1A and M79 (mAb M79 recognizes a different epitope on the 17-1A antigen) both could bind P33 and P50, but only M79 bound to P65 in an enzyme-linked immunosorbant assay (ELISA). These results indicate that P33 and P50 share at least two epitopes, and a common immunological epitope exists among P33, P50 and P65, suggesting that the two new proteins (P50 and P65) are related to the tumor-associated antigen 17-1A. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:101 / 105
页数:5
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