In vitro bioactivity, cytocompatibility, and antibiotic release profile of gentamicin sulfate-loaded borate bioactive glass/chitosan composites

被引：32

作者：

Cui, Xu ^{[1
]}

Gu, Yifei ^{[1
]}

Li, Le ^{[1
]}

Wang, Hui ^{[1
]}

Xie, Zhongping ^{[2
]}

Luo, Shihua ^{[2
]}

Zhou, Nai ^{[1
]}

Huang, Wenhai ^{[1
]}

Rahaman, Mohamed N. ^{[3
]}

机构：

[1] Tongji Univ, Inst Bioengn & Informat Technol Mat, Shanghai 200092, Peoples R China

[2] Jiaotong Univ, Shanghai Peoples Hosp 6, Dept Orthopaed Surg, Shanghai 200233, Peoples R China

[3] Missouri Univ Sci & Technol, Ctr Bone & Tissue Repair & Regenerat, Dept Mat Sci & Engn, Rolla, MO 65409 USA

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2013年 / 24卷 / 10期

基金：

中国国家自然科学基金;

关键词：

BOROSILICATE GLASSES; BORIC-ACID; BONE; SCAFFOLDS; BORON; HYDROXYAPATITE; TEICOPLANIN; MECHANISMS; SILICATE;

D O I：

10.1007/s10856-013-4996-0

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Borate bioactive glass-based composites have been attracting interest recently as an osteoconductive carrier material for local antibiotic delivery. In the present study, composites composed of borate bioactive glass particles bonded with a chitosan matrix were prepared and evaluated in vitro as a carrier for gentamicin sulfate. The bioactivity, degradation, drug release profile, and compressive strength of the composite carrier system were studied as a function of immersion time in phosphate-buffered saline at 37 A degrees C. The cytocompatibility of the gentamicin sulfate-loaded composite carrier was evaluated using assays of cell proliferation and alkaline phosphatase activity of osteogenic MC3T3-E1 cells. Sustained release of gentamicin sulfate occurred over similar to 28 days in PBS, while the bioactive glass converted continuously to hydroxyapatite. The compressive strength of the composite loaded with gentamicin sulfate decreased from the as-fabricated value of 24 +/- A 3 MPa to similar to 8 MPa after immersion for 14 days in PBS. Extracts of the soluble ionic products of the borate glass/chitosan composites enhanced the proliferation and alkaline phosphatase activity of MC3T3-E1 cells. These results indicate that the gentamicin sulfate-loaded composite composed of chitosan-bonded borate bioactive glass particles could be useful clinically as an osteoconductive carrier material for treating bone infection.

引用

页码：2391 / 2403

页数：13

共 45 条

[1]

In vivo and in vitro effects of boron and boronated compounds [J].