Immunohistochemical detection of Bax and Bcl-2 proteins in gerbil hippocampus following transient forebrain ischemia

被引：85

作者：

Hara, A

Iwai, T

Niwa, M

Uematsu, T

Yoshimi, N

Tanaka, T

Mori, H

机构：

[1] ASAHI UNIV, MURAKAMI MEM HOSP, DEPT NEUROSURG, GIFU, JAPAN

[2] GIFU UNIV, SCH MED, DEPT PHARMACOL, GIFU, JAPAN

来源：

BRAIN RESEARCH | 1996年 / 711卷 / 1-2期

关键词：

Bax; Bcl-2; CA1; gerbil; apoptosis; delayed neuronal death;

D O I：

10.1016/0006-8993(95)01436-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Time-course expression of Bax and Bcl-2 proteins, identified as apoptosis-regulating molecules, was assessed in gerbil hippocampus following transient forebrain ischemia. Brain sections from animals sacrificed at 48, 72, 96 h and 7 days following 5 min ischemia were immunohistochemically evaluated using polyclonal antibodies specific for Bax and Bcl-2 proteins, respectively. The intensity of Bax expression in CA1 neurons increased with time and peaked at 72 h, and immediately disappeared at 96 h following 5 min ischemia. No expression of Bcl-2 in the CA1 neurons was recognized in all the time evaluated.

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页码：249 / 253

页数：5

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