Detection of low dose radiation induced DNA damage using temperature differential fluorescence assay

被引：24

作者：

Rogers, KR

Apostol, A

Madsen, SJ

Spencer, CW

机构：

[1] US EPA, Natl Exposure Res Lab, Las Vegas, NV 89193 USA

[2] Univ Nevada, Dept Hlth Phys, Las Vegas, NV 89154 USA

[3] Nevada Radiat Oncol Ctr, Las Vegas, NV 89106 USA

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 19期

关键词：

D O I：

10.1021/ac990537z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and sensitive fluorescence assay for radiation-induced DNA damage is reported. Changes in temperature-induced strand separation in both calf thymus DNA and plasmid DNA (puc 19 plasmid from Escherichia coli) were measured after exposure to low doses of radiation. Exposures of between 0.004 and 1 Gy were measured with doses as low as 0.008 Gy yielding significant responses. The double-strand, sensitive dye PicoGreen was used as an indicator of DNA denaturation. Calibration plots indicate that fluorescence changes corresponding to amounts as low as 1 ng of double stranded DNA (10(6) copies for plasmid puc 19) are detected by this method.

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页码：4423 / 4426

页数：4

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