Investigation of the yvgW Bacillus subtilis chromosomal gene involved in Cd2+ ion resistance

被引：24

作者：

Solovieva, IM

Entian, KD

机构：

[1] Univ Frankfurt, Inst Microbiol, D-60439 Frankfurt, Germany

[2] Russian Acad Sci, St Petersburg Nucl Phys Inst, Mol & Radiat Biophys Div, Gatchina 188350, St Petersburg, Russia

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 208卷 / 01期

关键词：

cadmium resistance; gene deletion; Bacillus subtilis;

D O I：

10.1016/S0378-1097(01)00587-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Analysis of the complete genome sequence of Bacillus subtilis has identified the gene yvgW encoding a protein of 703 amino acids with sequence similarity to the cadmium resistance determinant CadA from the Staphylococcus aureus plasmid pI258. Deletion of yvgW (designated cadA) resulted in increased sensitivity of the strain to cadmium. The cadA gene is expressed from its own promoter, and its expression is induced by cadmium. Northern hybridization analysis showed that cadmium induces the synthesis of a 2.2-kb cadA transcript. These results indicate that cadA is the chromosomal determinant to cadmium resistance in B. subtilis. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

收藏

页码：105 / 109

页数：5

相关论文

共 20 条

[1] REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS [J].

ANAGNOSTOPOULOS, C ;

SPIZIZEN, J .

JOURNAL OF BACTERIOLOGY, 1961, 81 (05) :741-&

[2] Properties of the P-type ATPases encoded by the copAP operons of Helicobacter pylori and Helicobacter felis [J].

Bayle, D ;

Wängler, S ;

Weitzenegger, T ;

Steinhilber, W ;

Volz, J ;

Przybylski, M ;

Schäfer, KP ;

Sachs, G ;

Melchers, K .

JOURNAL OF BACTERIOLOGY, 1998, 180 (02) :317-329

[3] Cloning and expression of cadD, a new cadmium resistance gene of Staphylococcus aureus [J].

Crupper, SS ;

Worrell, V ;

Stewart, GC ;

Iandolo, JJ .

JOURNAL OF BACTERIOLOGY, 1999, 181 (13) :4071-4075

[4] CADC, THE TRANSCRIPTIONAL REGULATORY PROTEIN OF THE CADMIUM RESISTANCE SYSTEM OF STAPHYLOCOCCUS-AUREUS PLASMID PI258 [J].

ENDO, G ;

SILVER, S .

JOURNAL OF BACTERIOLOGY, 1995, 177 (15) :4437-4441

[5] THE CADC GENE-PRODUCT OF ALKALIPHILIC BACILLUS-FIRMUS OF4 PARTIALLY RESTORES NA+ RESISTANCE TO AN ESCHERICHIA-COLI STRAIN LACKING AN NA+/H+ ANTIPORTER (NHAA) [J].

IVEY, DM ;

GUFFANTI, AA ;

SHEN, ZH ;

KUDYAN, N ;

KRULWICH, TA .

JOURNAL OF BACTERIOLOGY, 1992, 174 (15) :4878-4884

[6] REGULATION AND EXPRESSION OF THE ARSENIC RESISTANCE OPERON FROM STAPHYLOCOCCUS-AUREUS PLASMID PI258 [J].

JI, GY ;

SILVER, S .

JOURNAL OF BACTERIOLOGY, 1992, 174 (11) :3684-3694

[7] The complete genome sequence of the Gram-positive bacterium Bacillus subtilis [J].

Kunst, F ;

Ogasawara, N ;

Moszer, I ;

Albertini, AM ;

Alloni, G ;

Azevedo, V ;

Bertero, MG ;

Bessieres, P ;

Bolotin, A ;

Borchert, S ;

Borriss, R ;

Boursier, L ;

Brans, A ;

Braun, M ;

Brignell, SC ;

Bron, S ;

Brouillet, S ;

Bruschi, CV ;

Caldwell, B ;

Capuano, V ;

Carter, NM ;

Choi, SK ;

Codani, JJ ;

Connerton, IF ;

Cummings, NJ ;

Daniel, RA ;

Denizot, F ;

Devine, KM ;

Dusterhoft, A ;

Ehrlich, SD ;

Emmerson, PT ;

Entian, KD ;

Errington, J ;

Fabret, C ;

Ferrari, E ;

Foulger, D ;

Fritz, C ;

Fujita, M ;

Fujita, Y ;

Fuma, S ;

Galizzi, A ;

Galleron, N ;

Ghim, SY ;

Glaser, P ;

Goffeau, A ;

Golightly, EJ ;

Grandi, G ;

Guiseppi, G ;

Guy, BJ ;

Haga, K .

NATURE, 1997, 390 (6657) :249-256

[8] PLASMID-BORNE CADMIUM RESISTANCE GENES IN LISTERIA-MONOCYTOGENES ARE SIMILAR TO CADA AND CADC OF STAPHYLOCOCCUS-AUREUS AND ARE INDUCED BY CADMIUM [J].

LEBRUN, M ;

AUDURIER, A ;

COSSART, P .

JOURNAL OF BACTERIOLOGY, 1994, 176 (10) :3040-3048

[9]

MANDEL M, 1970, J MOL BIOL, V53, P154

[10]

Miller J. H., 1972, EXPT MOL GENETICS