MicroRNA-21 down-regulates the expression of tumor suppressor PDCD4 in human glioblastoma cell T98G

被引：211

作者：

Chen, Yang ^{[1
,2
,3
,4
]}

Liu, Wei ^{[3
]}

Chao, Tengfei ^{[3
,4
]}

Zhang, Yu ^{[3
,4
]}

Yan, Xingqi ^{[3
,4
]}

Gong, Yanhua ^{[3
]}

Qiang, Boqin ^{[3
]}

Yuan, Jiangang ^{[3
]}

Sun, Maosheng ^{[1
,2
]}

Peng, Xiaozhong ^{[3
]}

机构：

[1] Chinese Acad Med Sci, Inst Med Biol, Dept Biochem & Mol Biol, Kunming 650118, Peoples R China

[2] Peking Union Med Coll, Kunming 650118, Peoples R China

[3] Chinese Acad Med Sci, Inst Basic Med Sci, Natl Lab Med Mol Biol, Beijing 100005, Peoples R China

[4] Tsinghua Univ, Peking Union Med Coll, Grad Sch, Beijing 100005, Peoples R China

来源：

CANCER LETTERS | 2008年 / 272卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Glioblastomas; miR-21; PDCD4; T98G;

D O I：

10.1016/j.canlet.2008.06.034

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

MicroRNAs have been linked to different cancer-related processes. The microRNA miR-21 appears to function as an anti-apoptosis factor in glioblastomas. However, the functional target genes of miR-21 are largely unknown in glioblastomas. In this study, bioinformatics analysis was used to identify miR-21 target sites in various genes. Luciferase activity assay showed that a number of genes involved in apoptosis, PDCD4, MTAP, and SOX5, carry putative miR-21 binding sites. Expression of PDCD4 protein correlates inversely with expression of miR-21 in a number of human glioblastoma cell lines such as T98G, A172, U87, and U251. Inhibition of miR-21 increases endogenous levels of PDCD4 in cell line T98G and over-expression miR-21 inhibits PDCD4-dependent apoptosis. Together, these results indicate that miR-21 expression plays a key role in regulating cellular processes in glioblastomas and may serve as a target for effective therapies. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：197 / 205

页数：9

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