SCFTIR1/AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism

被引：135

作者：

Baster, Pawel ^{[1
,2
]}

Robert, Stephanie ^{[1
,2
,3
]}

Kleine-Vehn, Jurgen ^{[1
,2
,4
]}

Vanneste, Steffen ^{[1
,2
]}

Kania, Urszula ^{[1
,2
]}

Grunewald, Wim ^{[1
,2
]}

De Rybel, Bert ^{[1
,2
,5
]}

Beeckman, Tom ^{[1
,2
]}

Friml, Jiri ^{[1
,2
,6
]}

机构：

[1] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[2] Univ Ghent, Dept Plant Biotechnol & Genet, B-9000 Ghent, Belgium

[3] Swedish Univ Agr Sci, Umea Plant Sci Ctr, Dept Forest Genet & Plant Physiol, S-90183 Umea, Sweden

[4] Univ Appl Life Sci & Nat Resources BOKU, Dept Appl Genet & Cell Biol, Vienna, Austria

[5] Wageningen Univ, Biochem Lab, NL-6700 AP Wageningen, Netherlands

[6] Inst Sci & Technol Austria IST Austria, Klosterneuburg, Austria

来源：

EMBO JOURNAL | 2013年 / 32卷 / 02期

关键词：

auxin; degradation; gravitropism; PIN; YUCCA FLAVIN MONOOXYGENASES; BOX PROTEIN TIR1; EFFLUX CARRIER; PLASMA-MEMBRANE; BINDING-PROTEIN; DEPENDENT ENDOCYTOSIS; RESPONSE FACTORS; BREFELDIN-A; HAIR-CELLS; ARABIDOPSIS;

D O I：

10.1038/emboj.2012.310

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The distribution of the phytohormone auxin regulates many aspects of plant development including growth response to gravity. Gravitropic root curvature involves coordinated and asymmetric cell elongation between the lower and upper side of the root, mediated by differential cellular auxin levels. The asymmetry in the auxin distribution is established and maintained by a spatio-temporal regulation of the PIN-FORMED (PIN) auxin transporter activity. We provide novel insights into the complex regulation of PIN abundance and activity during root gravitropism. We show that PIN2 turnover is differentially regulated on the upper and lower side of gravistimulated roots by distinct but partially overlapping auxin feedback mechanisms. In addition to regulating transcription and clathrin-mediated internalization, auxin also controls PIN abundance at the plasma membrane by promoting their vacuolar targeting and degradation. This effect of elevated auxin levels requires the activity of SKP-Cullin-F-box(TIR1/AFB) (SCFTIR1/AFB)-dependent pathway. Importantly, also suboptimal auxin levels mediate PIN degradation utilizing the same signalling pathway. These feedback mechanisms are functionally important during gravitropic response and ensure fine-tuning of auxin fluxes for maintaining as well as terminating asymmetric growth. The EMBO Journal (2013) 32, 260-274. doi:10.1038/emboj.2012.310; Published online 4 December 2012

引用

页码：260 / 274

页数：15

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