High throughput screening of signal transduction mutants with luciferase imaging

被引：20

作者：

Xiong, LM ^{[1
]}

David, L ^{[1
]}

Stevenson, B ^{[1
]}

Zhu, JK ^{[1
]}

机构：

[1] Univ Arizona, Dept Plant Sci, Tucson, AZ 85721 USA

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1999年 / 17卷 / 02期

关键词：

CCD camera; imaging; luciferase; mutant screening; signal transduction;

D O I：

10.1023/A:1007519200505

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A detailed procedure for high throughput genetic screening of hormone and environmental stress signal transduction mutants of Ambidopsis thaliana is described. The screen was carried out with mutagenized plants expressing the firefly luciferase reporter under control of a cold, osmotic stress, and absciscic acid responsive promoter. A thermoelectrically cooled CCD camera was used to detect luminescence emitted by the plants in response to stresses or ABA. Advantages of the screening procedure include high throughput, capability to identify low as well as high expression mutants and employment of a highly sensitive but affordable imaging system and software. This procedure can be used to study complex signal transduction networks in higher plants.

引用

页码：159 / 170

页数：12

共 7 条

[1] Genetic analysis of osmotic and cold stress signal transduction in Arabidopsis: Interactions and convergence of abscisic acid-dependent and abscisic acid-independent pathways [J].