共 49 条
Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-dependent RNA polymerase
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作者:

Vaistij, FE
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John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Jones, L
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John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Baulcombe, DC
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John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
机构:
[1] John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
来源:
关键词:
D O I:
10.1105/tpc.010480
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
RNA silencing is a sequence-specific RNA degradation process that follows the recognition of double-stranded RNA. Here, we show that virus vectors carrying parts of a green fluorescent protein (GFP) transgene targeted RNA silencing in Nicotiana benthamiana and Arabidopsis against the entire GFP RNA. These results indicate that there was spreading of RNA targeting from the initiator region into the adjacent 5' and 3' regions of the target gene. Spreading was accompanied by methylation of the corresponding GFP DNA. It also was dependent on transcription of the transgene and on the putative RNA-dependent RNA polymerase, SDE1/SGS2. These findings indicate that SDE1/SGS2 produces double-stranded RNA using the target RNA as a template. RNA silencing of ribulose-1,5-bisphosphate carboxylase/oxygenase and phytoene desaturase was not associated with the spreading of RNA targeting or DNA methylation, indicating that these endogenous RNAs are not templates for SDE1/SGS2.
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页码:857 / 867
页数:11
相关论文
共 49 条
[1]
Transcriptional and posttranscriptional plant gene silencing in response to a pathogen
[J].
Al-Kaff, NS
;
Covey, SN
;
Kreike, MM
;
Page, AM
;
Pinder, R
;
Dale, PJ
.
SCIENCE,
1998, 279 (5359)
:2113-2115

Al-Kaff, NS
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

Covey, SN
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

Kreike, MM
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

Page, AM
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

Pinder, R
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

Dale, PJ
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England
[2]
Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D-melanogaster germline
[J].
Aravin, AA
;
Naumova, NM
;
Tulin, AV
;
Vagin, VV
;
Rozovsky, YM
;
Gvozdev, VA
.
CURRENT BIOLOGY,
2001, 11 (13)
:1017-1027

Aravin, AA
论文数: 0 引用数: 0
h-index: 0
机构: Inst Mol Genet, Dept Anim Mol Genet, Moscow 123182, Russia

Naumova, NM
论文数: 0 引用数: 0
h-index: 0
机构: Inst Mol Genet, Dept Anim Mol Genet, Moscow 123182, Russia

Tulin, AV
论文数: 0 引用数: 0
h-index: 0
机构: Inst Mol Genet, Dept Anim Mol Genet, Moscow 123182, Russia

Vagin, VV
论文数: 0 引用数: 0
h-index: 0
机构: Inst Mol Genet, Dept Anim Mol Genet, Moscow 123182, Russia

Rozovsky, YM
论文数: 0 引用数: 0
h-index: 0
机构: Inst Mol Genet, Dept Anim Mol Genet, Moscow 123182, Russia

Gvozdev, VA
论文数: 0 引用数: 0
h-index: 0
机构:
Inst Mol Genet, Dept Anim Mol Genet, Moscow 123182, Russia Inst Mol Genet, Dept Anim Mol Genet, Moscow 123182, Russia
[3]
Gene silencing: RNA makes RNA makes no protein
[J].
Baulcombe, DC
.
CURRENT BIOLOGY,
1999, 9 (16)
:R599-R601

Baulcombe, DC
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
[4]
Role for a bidentate ribonuclease in the initiation step of RNA interference
[J].
Bernstein, E
;
Caudy, AA
;
Hammond, SM
;
Hannon, GJ
.
NATURE,
2001, 409 (6818)
:363-366

Bernstein, E
论文数: 0 引用数: 0
h-index: 0
机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Caudy, AA
论文数: 0 引用数: 0
h-index: 0
机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Hammond, SM
论文数: 0 引用数: 0
h-index: 0
机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Hannon, GJ
论文数: 0 引用数: 0
h-index: 0
机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
[5]
Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana
[J].
Chuang, CF
;
Meyerowitz, EM
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
2000, 97 (09)
:4985-4990

Chuang, CF
论文数: 0 引用数: 0
h-index: 0
机构:
CALTECH, Dept Biol 156 29, Pasadena, CA 91125 USA CALTECH, Dept Biol 156 29, Pasadena, CA 91125 USA

Meyerowitz, EM
论文数: 0 引用数: 0
h-index: 0
机构:
CALTECH, Dept Biol 156 29, Pasadena, CA 91125 USA CALTECH, Dept Biol 156 29, Pasadena, CA 91125 USA
[6]
Plants combat infection by gene silencing
[J].
Covey, SN
;
AlKaff, NS
;
Langara, A
;
Turner, DS
.
NATURE,
1997, 385 (6619)
:781-782

Covey, SN
论文数: 0 引用数: 0
h-index: 0
机构: Department of Virus Research, John Innes Centre, Colney, Norwich NR4 7UH, Norwich Research Park

AlKaff, NS
论文数: 0 引用数: 0
h-index: 0
机构: Department of Virus Research, John Innes Centre, Colney, Norwich NR4 7UH, Norwich Research Park

Langara, A
论文数: 0 引用数: 0
h-index: 0
机构: Department of Virus Research, John Innes Centre, Colney, Norwich NR4 7UH, Norwich Research Park

Turner, DS
论文数: 0 引用数: 0
h-index: 0
机构: Department of Virus Research, John Innes Centre, Colney, Norwich NR4 7UH, Norwich Research Park
[7]
Potato virus X amplicons in arabidopsis mediate genetic and epigenetic gene silencing
[J].
Dalmay, T
;
Hamilton, A
;
Mueller, E
;
Baulcombe, DC
.
PLANT CELL,
2000, 12 (03)
:369-379

Dalmay, T
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Hamilton, A
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Mueller, E
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Baulcombe, DC
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Inst, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
[8]
An RNA-Dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus
[J].
Dalmay, T
;
Hamilton, A
;
Rudd, S
;
Angell, S
;
Baulcombe, DC
.
CELL,
2000, 101 (05)
:543-553

Dalmay, T
论文数: 0 引用数: 0
h-index: 0
机构: John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Hamilton, A
论文数: 0 引用数: 0
h-index: 0
机构: John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Rudd, S
论文数: 0 引用数: 0
h-index: 0
机构: John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Angell, S
论文数: 0 引用数: 0
h-index: 0
机构: John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Baulcombe, DC
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
[9]
SDE3 encodes an RNA helicase required for posttranscriptional gene silencing in Arabidopsis
[J].
Dalmay, T
;
Horsefield, R
;
Braunstein, TH
;
Baulcombe, DC
.
EMBO JOURNAL,
2001, 20 (08)
:2069-2077

Dalmay, T
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Horsefield, R
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Braunstein, TH
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

Baulcombe, DC
论文数: 0 引用数: 0
h-index: 0
机构:
John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
[10]
RNA interference is mediated by 21-and 22-nucleotide RNAs
[J].
Elbashir, SM
;
Lendeckel, W
;
Tuschl, T
.
GENES & DEVELOPMENT,
2001, 15 (02)
:188-200

Elbashir, SM
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biophys Chem, Dept Cellular Biochem, D-37077 Gottingen, Germany Max Planck Inst Biophys Chem, Dept Cellular Biochem, D-37077 Gottingen, Germany

Lendeckel, W
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biophys Chem, Dept Cellular Biochem, D-37077 Gottingen, Germany Max Planck Inst Biophys Chem, Dept Cellular Biochem, D-37077 Gottingen, Germany

Tuschl, T
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biophys Chem, Dept Cellular Biochem, D-37077 Gottingen, Germany Max Planck Inst Biophys Chem, Dept Cellular Biochem, D-37077 Gottingen, Germany