Inexpensive isolation Of β-D-glucopyranosidase from α-L-arabinofuranosidase, α-L-rhamnopyranosidase, and o-acetylesterase

被引：10

作者：

Barbagallo, RN ^{[1
]}

Spagna, G ^{[1
]}

Abbate, C ^{[1
]}

Azzaro, G ^{[1
]}

Palmeri, R ^{[1
]}

机构：

[1] Univ Catania, Biotechnol Grp, Dept Hort Floriculture Arboriculture & Agroind Te, DOFATA, I-95123 Catania, Italy

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2002年 / 101卷 / 01期

关键词：

beta-D-glucopyranosidase isolation; alpha-L-arabinofuranosidase; alpha-L-rhamnopyranosidase; o-acetylesterase; brown substances and polysaccharides;

D O I：

10.1385/ABAB:101:1:01

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-D-Glucopyranosidase (betaG, EC 3.2.1.21) has been isolated from some collateral activities, alpha-L-arabinofuranosidase (Ara, EC 3.2.1.55), alpha-L-rhamnopyranosidase (Rha, EC 3.2.1.40), and o-acetylesterase (Est, EC 3.1.1.53), using a commercial enzyme preparation and a simple method economically sustainable for the food industry. The procedure comprises precipitation of extraneous substances by adding ethanol and CaCl2. ultrafiltration, and adsorption, first on bentonite and then on chitosan. The results obtained were the complete isolation of betaG from the above-mentioned activities, a drastic reduction in extraneous compounds, such as brown substances and polysaccharides, and a slight increase in purification.

引用

页码：1 / 13

页数：13

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