Occurrence of Coxiella burnetii in goat and ewe unpasteurized cheeses: Screening and genotyping

被引：27

作者：

Galiero, Alessia ^{[1
]}

Fratini, Filippo ^{[1
]}

Camma, Cesare ^{[2
]}

Di Domenico, Marco ^{[2
]}

Curini, Valentina ^{[2
]}

Baronti, Irene ^{[1
]}

Turchi, Barbara ^{[1
]}

Cerri, Domenico ^{[1
]}

机构：

[1] Univ Pisa, Dept Vet Sci, Viale Piagge 2, I-56124 Pisa, Italy

[2] Ist Zooprofilatt Sperimentale Abruzzo & Molise G, Via Campo Boario, I-64100 Teramo, Italy

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2016年 / 237卷

关键词：

Coxiella burnetii; Cheese; Small ruminants; PCR; Multispacer sequence typing; Tuscany; Q-FEVER; DAIRY-PRODUCTS; BULK MILK; RAW-MILK; ABORTION; PREVALENCE; SHEEP; PATHOGENS; OUTBREAK; BOVINE;

D O I：

10.1016/j.ijfoodmicro.2016.08.008

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Q fever is a zoonosis caused by Coxiella burnetii which infects humans as well as several animal species; sheep, goats and cattle are the primary animal reservoir. The main route of human exposure to Coxiella burnetii is inhalation of contaminated aerosols from excreta, especially birth products, while the role of unpasteurized dairy products in the transmission of Q fever to humans remains still controversial. The aim of this work was to evaluate the presence of Coxiella burnetii in unpasteurized cheese samples (n = 84) by PCR and to genotype the circulating strains by Multispacer sequence typing (MST) analysis. Coxiella burnetii DNA was detected in 27/84 (32.14%) cheeses and positivity rate of handicraft cheeses reached 17.24%, while positivity rate of non-handicraft cheeses reached 65.38%. In addition, the MST profile of Coxiella burnetii detected in 5 cheese samples have shown the circulation of ST12 and ST32 genotypes in Tuscany. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：47 / 54

页数：8

共 61 条

[1]

Aquilini D., 2000, J Spirochetal Tick-Borne Dis, V7, P35

[2] Is Q Fever an emerging or re-emerging zoonosis? [J].

Arricau-Bouvery, N ;

Rodolakis, A .

VETERINARY RESEARCH, 2005, 36 (03) :327-349

[3] Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing [J].

Arricau-Bouvery, Nathalie ;

Hauck, Yolande ;

Bejaoui, Awatef ;

Frangoulidis, Dimitrios ;

Bodier, Christelle C. ;

Souriau, Armel ;

Meyer, Hermann ;

Neubauer, Heinrich ;

Rodolakis, Annie ;

Vergnaud, Gilles .

BMC MICROBIOLOGY, 2006, 6 (1)

[4] Kinetics of Coxiella burnetii excretion in a commercial dairy sheep flock after treatment with oxytetracycline [J].

Astobiza, Ianire ;

Barandika, Jesus F. ;

Hurtado, Ana ;

Juste, Ramon A. ;

Garcia-Perez, Ana L. .

VETERINARY JOURNAL, 2010, 184 (02) :172-175

[5]

BABUDIERI B., 1950, REND IST SUPERIORE SANITA [ROME], V13, P739

[6] Estimated herd prevalence and sequence types of Coxiella burnetii in bulk tank milk samples from commercial dairies in Indiana [J].

Bauer, Amy E. ;

Olivas, Sonora ;

Cooper, Maria ;

Hornstra, Heidie ;

Keim, Paul ;

Pearson, Talima ;

Johnson, April J. .

BMC VETERINARY RESEARCH, 2015, 11

[7]

Borriello G, 2010, LARGE ANIM REV, V16, P273

[8] Scientific Opinion on Q fever EFSA Panel on Animal Health and Welfare (AHAW) EFSA Panel on Biological Hazards (BIOHAZ) (Chapter 4 on Food Safety) [J].

Botner, Anette ;

Broom, Donald ;

Doherr, Marcus G. ;

Domingo, Mariano ;

Hartung, Joerg ;

Keeling, Linda ;

Koenen, Frank ;

More, Simon ;

Morton, David ;

Oltenacu, Pascal ;

Osterhaus, Albert ;

Salati, Fulvio ;

Salman, Mo ;

Sanaa, Moez ;

Sharp, James M. ;

Stegeman, Jan A. ;

Szuecs, Endre ;

Thulke, Hans-H. ;

Vannier, Philippe ;

Webster, John ;

Wierup, Martin ;

Andreoletti, Olivier ;

Budka, Herbert ;

Buncic, Sava ;

Collins, John D. ;

Griffin, John ;

Havelaar, Arie ;

Hope, James ;

Klein, Guenter ;

Hald, Tine ;

Messens, Winy ;

McLauchlin, James ;

Mueller-Graf, Christine ;

Nguyen-The, Christophe ;

Noerrung, Birgit ;

Maradona, Miguel Prieto ;

Peixe, Luisa ;

Ricci, Antonia ;

Sofos, John ;

Threlfall, John ;

Vagsholm, Ivar ;

Vanopdenbosch, Emmanuel .

EFSA JOURNAL, 2010, 8 (05)

[9] A multiplex polymerase chain reaction for the identification of cows', goats' and sheep's milk in dairy products [J].

Bottero, MT ;

Civera, T ;

Nucera, D ;

Rosati, S ;

Sacchi, P ;

Turi, RM .

INTERNATIONAL DAIRY JOURNAL, 2003, 13 (04) :277-282

[10]

Cabassi CS, 2006, NEW MICROBIOL, V29, P211

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