A novel approach for isolation of volatile chemicals released by individual leaves of a plant in situ

被引:82
作者
Agelopoulos, NG [1 ]
Hooper, AM [1 ]
Maniar, SP [1 ]
Pickett, JA [1 ]
Wadhams, LJ [1 ]
机构
[1] IACR Rothamsted, Biol & Ecol Chem Dept, Harpenden AL5 2JQ, Herts, England
基金
英国生物技术与生命科学研究理事会;
关键词
volatile collection chamber; mechanical leaf damage; Solanum tuberosum; Vicia faba; sesquiterpenes; green leaf volatiles;
D O I
10.1023/A:1020939112234
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A glass chamber designed specifically for collecting volatile chemicals from individual leaves of a plant in situ is described. The effectiveness of the chamber was demonstrated by collecting volatile chemicals from single leaves of two plant species, potato (Solanum tuberosum) and broad bean (Vicia faba), before and after mechanical damage. The glass chamber, in conjunction with thermal desorption, enables reduction of the entrainment time and thereby allows the monitoring of compounds released by leaf damage in successive 5-min periods. An intact broad bean leaf, in the middle of the day, produces small amounts of the green leaf volatiles (E)-2-hexenal and (Z)-3-hexen-1-ol. However, during the first 5 min after mechanical damage, large amounts of (Z)-3-hexenal, (E)-2-hexenal, and (Z)-3-hexen-1-ol are produced. The decline in production of (Z)-3-hexenal and (E)-2-hexenal is fast, and after 10 min, these compounds reach very low levels. (Z)-3-Hexen-1-ol shows an increase for the first 10 min and then a gradual decline. An intact potato leaf, in the middle of the day, produces very small amounts of the sesquiterpene hydrocarbons P-caryophyllene and germacrene-D. After being damaged, the profile of released volatiles is different from that of broad bean. In potato, damage is associated with release of large amounts of green leaf volatiles and sesquiterpene hydrocarbons. Compounds such as (Z)-3-hexenal, (E)-2-hexenal, and (Z)-3-hexen-1-ol are released in high amounts during the first 5 min after damage, but after 10 min, these drop to very low levels. High release associated with damage is also observed for beta-caryophyllene, (E)-beta-farnesene, germacrene-D, and beta-bisabolene. The highest level is reached 5 min after damage and 15 min later, these compounds drop to low levels. The significance of compounds released after plant damage is discussed.
引用
收藏
页码:1411 / 1425
页数:15
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