The di-leucine motif of vesicle-associated membrane protein 4 is required for its localization and AP-1 binding

被引：72

作者：

Peden, AA

Park, GY

Scheller, RH ^{[1
]}

机构：

[1] Genentech Inc, San Francisco, CA 94080 USA

[2] Stanford Univ, Howard Hughes Med Inst, Sch Med, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 52期

关键词：

D O I：

10.1074/jbc.M106646200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heterotetrameric adaptor complexes and SNAREs play key roles in the specificity of membrane budding and fusion. Here we test the hypothesis that vesicle budding and membrane fusion are coupled by the interaction of these molecules. We investigate the role of the di-leucine motif of vesicle-associated membrane protein 4 (VAMP4) in adaptor binding and localization of VAMP4. Mutation of the di-leucine motif inhibits AP-1 binding in vitro and affects the steady state distribution of VAMP4 in vivo.

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收藏

页码：49183 / 49187

页数：5

相关论文

共 24 条

[1] VAMP-7 mediates vesicular transport from endosomes to lysosomes [J].

Advani, RJ ;

Yang, B ;

Prekeris, R ;

Lee, KC ;

Klumperman, J ;

Scheller, RH .

JOURNAL OF CELL BIOLOGY, 1999, 146 (04) :765-775

[2] PROTEIN-PROTEIN INTERACTIONS CONTRIBUTING TO THE SPECIFICITY OF INTRACELLULAR VESICULAR TRAFFICKING [J].

CALAKOS, N ;

BENNETT, MK ;

PETERSON, KE ;

SCHELLER, RH .

SCIENCE, 1994, 263 (5150) :1146-1149

[3] Acidic di-leucine motif essential for AP-3-dependent sorting and restriction of the functional specificity of the Vam3p vacuolar t-SNARE [J].

Darsow, T ;

Burd, CG ;

Emr, SD .

JOURNAL OF CELL BIOLOGY, 1998, 142 (04) :913-922

[4] Syntaxin 6 functions in trans-Golgi network vesicle trafficking [J].

Davanger, S ;

Bock, JB ;

Klumperman, J ;

Scheller, RH .

MOLECULAR BIOLOGY OF THE CELL, 1997, 8 (07) :1261-1271

[5] Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells [J].

Hay, JC ;

Chao, DS ;

Kuo, CS ;

Scheller, RH .

CELL, 1997, 89 (01) :149-158

[6] SNAREs and NSF in targeted membrane fusion [J].

Hay, JC ;

Scheller, RH .

CURRENT OPINION IN CELL BIOLOGY, 1997, 9 (04) :505-512

[7] Clathrin and adaptors [J].

Hirst, J ;

Robinson, MS .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1998, 1404 (1-2) :173-193

[8]

MANFREDI JJ, 1987, J BIOL CHEM, V262, P12182

[9] μ1A-adaptin-deficient mice:: lethality, loss of AP-1 binding and rerouting of mannose 6-phosphate receptors [J].

Meyer, C ;

Zizioli, D ;

Lausmann, S ;

Eskelinen, EL ;

Hamann, J ;

Saftig, P ;

von Figura, K ;

Schu, P .

EMBO JOURNAL, 2000, 19 (10) :2193-2203

[10] The sortilin cytoplasmic tail conveys Golgi-endosome transport and binds the VHS domain of the GGA2 sorting protein [J].

Nielsen, MS ;

Madsen, P ;

Christensen, EI ;

Nykjær, A ;

Gliemann, J ;

Kasper, D ;

Pohlmann, R ;

Petersen, CM .

EMBO JOURNAL, 2001, 20 (09) :2180-2190