Leptospira weilii serovar Topaz, a new member of the Tarassovi serogroup isolated from a bovine source in Queensland, Australia

被引:14
作者
Corney, B. G. [2 ]
Slack, A. T. [1 ]
Symonds, M. L. [1 ]
Dohnt, M. F. [1 ]
McClintock, C. S. [3 ]
McGowan, M. R. [3 ]
Smythe, L. D. [1 ]
机构
[1] Queensland Hlth Forens & Sci Serv, WHO FAO OIE Collaborating Ctr Reference & Res Lep, Brisbane, Qld, Australia
[2] Queensland Dept Primary Ind & Fisheries, Anim Res Inst, Yeerongpilly, Qld, Australia
[3] Univ Queensland, St Lucia, Qld, Australia
关键词
D O I
10.1099/ijs.0.64884-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This paper reports on a Leptospira isolate of bovine origin and its identification as belonging to a previously unknown serovar, for which the name Topaz is proposed. The isolate (94-79970/3) was cultured from bovine urine from a north Queensland dairy farm in Australia. Strain 94-79970/3 grew at 30 degrees C in Ellinghausen McCullough Johnson Harris (EMJH) medium but failed to grow at 13 degrees C in EMJH medium or in the presence of 8-azaguanine. Serologically, strain 94-79970/3 produced titres against the Leptospira borgpetersenii serovar Tarassovi, the reference strain for the Tarassovi serogroup; however, no significant titres to any other serovars within the serogroup were obtained. Using 16S rRNA and DNA gyrase subunit B gene analysis, strain 94-79970/3 was identified as a member of the species Leptospira weilii. We propose that the serovar be named Topaz, after the location where the original isolate was obtained. The reference strain for this serovar is 94-79970/3 (=KIT 94-79970/3=LT722).
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页码:2249 / 2252
页数:4
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