Cell proliferation is promoted by compressive stress during early stage of chondrogenic differentiation of rat BMSCs

被引：25

作者：

Wang, Yating ^{[1
]}

Wang, Jun ^{[1
]}

Bai, Ding ^{[1
]}

Song, Jinlin ^{[2
]}

Ye, Rui ^{[1
]}

Zhao, Zhihe ^{[1
]}

Lei, Lei ^{[1
]}

Hao, Jin ^{[1
]}

Jiang, Chunmiao ^{[1
]}

Fang, Shanbao ^{[1
]}

An, Shu ^{[1
]}

Cheng, Qian ^{[1
]}

Li, Juan ^{[1
]}

机构：

[1] Sichuan Univ, West China Sch Stomatol, West China Hosp Stomatol, Dept Orthodont,State Key Lab Oral Dis, Chengdu 610041, Peoples R China

[2] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Affiliated Hosp Stomatol, Chongqing 401147, Peoples R China

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2013年 / 228卷 / 09期

基金：

中国国家自然科学基金;

关键词：

MESENCHYMAL STEM-CELLS; CHONDROCYTE PROLIFERATION; II COLLAGEN; TGF-BETA; EXPRESSION; FORCE; BONE; MECHANOTRANSDUCTION; HYPERTROPHY; CARTILAGE;

D O I：

10.1002/jcp.24359

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

The presence of an appropriate number of viable cells is prerequisite for successive differentiation during chondrogenesis. Chondrogenic differentiation has been reported to be influenced by mechanical stimuli. This research aimed to study the effects of cyclic compressive stress on cell viability of rat bone marrow-derived MSCs (BMSCs) during chondrogenesis as well as its underlying mechanisms. The results showed that dynamic compression increased cell quantity and viability remarkably in the early stage of chondrogenesis, during which the expression of Ihh, Cyclin D1, CDK4, and Col21 were enhanced significantly. Possible signal pathways implicated in the process were explored in our study. MEK/ERK and p38 MAPK were not found to function in this process while BMP signaling seemed to play an important role in the mechanotransduction during chondrogenic proliferation. In conclusion, dynamic compressive stress could enhance cell viability during chondrogenesis, which might be achieved by activating BMP signaling. J. Cell. Physiol. 228: 19351942, 2013. (c) 2013 Wiley Periodicals, Inc.

引用

页码：1935 / 1942

页数：8

共 38 条

[1]

TGFβ and PTHrP control chondrocyte proliferation by activating cyclin D1 expression [J].