Binding assays with molecularly imprinted polymers - why do they work?

被引：23

作者：

Pap, T

Horvai, G

机构：

[1] Budapest Univ Technol & Econ, Hungarian Acad Sci, Res Grp Tech Analyt Chem, H-1111 Budapest, Hungary

[2] Budapest Univ Technol & Econ, Dept Chem Informat technol, H-1111 Budapest, Hungary

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 804卷 / 01期

关键词：

binding assays; molecularly imprinted polymers;

D O I：

10.1016/j.jchromb.2003.12.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The design of homologous displacement ligand binding assays based on molecularly imprinted polymers (MIP) is discussed in terms of the MIP adsorption isotherm. It is shown that only MIPs having a binding isotherm with varying slope are suitable for the assay, but there is no need to interpret the isotherm in terms of site affinity and population. One can calculate the calibration plot of the binding assay from the isotherm and vice versa. (C) 2003 Elsevier B.V. All rights reserved.

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页码：167 / 172

页数：6

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