Experimental periodontitis promotes transient vascular inflammation and endothelial dysfunction

被引:62
作者
Brito, Lorena C. W. [1 ]
DalBo, Silvia [2 ]
Striechen, Tatiane M. [3 ]
Farias, Jessica M. [3 ]
Olchanheski, Luiz R., Jr. [1 ]
Mendes, Reila T. [3 ]
Vellosa, Jose C. R. [4 ]
Favero, Giovani M. [5 ]
Sordi, Regina [2 ]
Assreuy, Jamil [2 ]
Santos, Fabio A. [3 ]
Fernandes, Daniel [1 ]
机构
[1] Univ Estadual Ponta Grossa, Dept Pharmaceut Sci, Ponta Grossa, PR, Brazil
[2] Univ Fed Santa Catarina, Dept Pharmacol, BR-88040900 Florianopolis, SC, Brazil
[3] Univ Estadual Ponta Grossa, Dept Dent, Ponta Grossa, PR, Brazil
[4] Univ Estadual Ponta Grossa, Dept Clin & Toxicol Anal, Ponta Grossa, PR, Brazil
[5] Univ Estadual Ponta Grossa, Dept Gen Biol, Ponta Grossa, PR, Brazil
关键词
Endothelial dysfunction; Periodontitis; Superoxide; Inflammation; Nitric oxide; Cholesterol; C-REACTIVE PROTEIN; CORONARY-HEART-DISEASE; NITRIC-OXIDE; MEDIATED VASODILATION; BRACHIAL-ARTERY; ANIMAL-MODELS; RISK; RESISTANCE; INFECTION; BACTERIA;
D O I
10.1016/j.archoralbio.2013.03.009
中图分类号
R78 [口腔科学];
学科分类号
100302 [口腔临床医学];
摘要
Objectives: This study aimed to evaluate the systemic inflammatory response and cardiovascular changes induced by experimental periodontitis in rats. Design: Experimental periodontitis was induced by placing a cotton ligature around the cervix of both sides of mandibular first molars and maxillary second molars in each male rat. Sham-operated rats had the ligature removed immediately after the procedure. Seven, 14 or 28 days after procedure, the effects of acetylcholine, sodium nitroprusside and phenylephrine were evaluated on blood pressure, aortic rings and isolated and perfused mesenteric bed. The blood was obtained for plasma Interleukin-6 (IL-6), C-reactive protein (CRP) and lipid evaluation. The mesenteric vessels were obtained to evaluate superoxide production and nitric oxide synthase 3 (NOS-3) expression. Results: Ligature induced periodontitis reduced endothelium-dependent vasodilatation, a hallmark of endothelial dysfunction. This effect was associated with an increase in systemic inflammatory markers (IL-6 and CRP), worsens on lipid profile, increased vascular superoxide production and reduced NOS-3 expression. It is interesting to note that many of these effects were transitory. Conclusion: Periodontitis induced a transient systemic and vascular inflammation which leads to endothelial dysfunction, an initial step for cardiovascular diseases. Moreover, the animal model of periodontitis used here may represent a valuable tool for studying the relationship between periodontitis and endothelial dysfunction. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1187 / 1198
页数:12
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