The U(L)16 gene product of herpes simplex virus 1 is a virion protein that colocalizes with intranuclear capsid proteins

The U(L)16 gene of herpes simplex virus maps within the intron of the U(L)15 gene. This report shows the following: (i) A polyclonal antiserum directed against a bacterial fusion protein containing glutathione S-transferase fused to the C-terminus of the U(L)16 gene reacted with an apparent M(r), 40,000 protein in HSV-1 infected cell lysates. (ii) The protein encoded by U(L)16 was dependent on viral DNA synthesis for accumulation to detectable levels. (iii) In immunofluorescence studies, the polyclonal U(L)16/GST-specific antiserum was shown to stain the nucleus of infected cells at 18 hr after infection in areas containing high concentrations of HSV capsid proteins. These nuclear compartments have been described previously as viral assemblons (Ward et at, J. Virol. 70, 4623-4631, 1996) end are distinct from compartments containing replicating DNA. Localization within assemblons argues far a role of U(L)16 encoded protein in capsid assembly or maturation. (iv) At 22 hr after infection, U(L)16-specific immunofluorescence was present in both the nucleus and the cytoplasm. (v) Consistent with the change in localization at late times after infection, the U(L)16 protein was found to be a component of purified virions. (C) 1996 Academic Press.