Hepatitis delta virus induces specific DNA methylation processes in Huh-7 liver cancer cells

被引：35

作者：

Benegiamo, Giorgia ^{[1
]}

Vinciguerra, Manlio ^{[2
]}

Guarnieri, Vito ^{[3
]}

Niro, Grazia Anna ^{[1
]}

Andriulli, Angelo ^{[1
]}

Pazienza, Valerio ^{[1
]}

机构：

[1] IRCCS Casa Sollievo della Sofferenza Hosp, Gastroenterol Unit, I-71013 San Giovanni Rotondo, FG, Italy

[2] UCL, Div Med, Inst Liver & Digest Hlth, London, England

[3] IRCCS Casa Sollievo della Sofferenza Hosp, I-71013 San Giovanni Rotondo, FG, Italy

来源：

FEBS LETTERS | 2013年 / 587卷 / 09期

关键词：

HDV; DNA methyltransferase; HCC; CORE-PROTEIN; E-CADHERIN; B-VIRUS; EXPRESSION; INFECTION; DNMT1; DNA-METHYLTRANSFERASE-1; TRANSCRIPTION; INITIATION; CARCINOMA;

D O I：

10.1016/j.febslet.2013.03.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Hepatitis delta virus (HDV) is a small, defective RNA virus that can infect only individuals carrying hepatitis B virus. HBV/HDV co-infection results in more severe liver disease than HBV single infection and more rapid progression to cirrhosis and hepatocellular carcinoma (HCC). The epigenetic events involved in hepatocyte transformation towards malignancy in this context are poorly known. Here we report that, in Huh-7 cells, HDV induces DNMT3b expression and is associated to E2F1 transcription factor hypermethylation. Moreover our cell cycle analysis showed that HDV induces G2/M arrest. These findings suggest that HDV could play a role in HCC development at least in part by altering DNA methylation events. A better understanding of the molecular mechanisms involved in HDV-related carcinogenesis could help to identify new therapeutic targets. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1424 / 1428

页数：5

共 37 条

[1]

Hepatitis C virus core protein downregulates E-cadherin expression via activation of DNA methyltransferase 1 and 3b [J].

Arora, Payal ;

Kim, Eun-Ok ;

Jung, Jin Kyu ;

Jang, Kyung Lib .

CANCER LETTERS, 2008, 261 (02) :244-252

[2]

DNA Methyltransferases 1 and 3b Expression in Huh-7 Cells Expressing HCV Core Protein of Different Genotypes [J].

Benegiamo, Giorgia ;

Vinciguerra, Manlio ;

Mazzoccoli, Gianluigi ;

Piepoli, Ada ;

Andriulli, Angelo ;

Pazienza, Valerio .

DIGESTIVE DISEASES AND SCIENCES, 2012, 57 (06) :1598-1603

[3]

Signal transducer and activator of transcription proteins in leukemias [J].

Benekli, M ;

Baer, MR ;

Baumann, H ;

Wetzler, M .

BLOOD, 2003, 101 (08) :2940-2954

[4]

CpG island methylation in precursors of gastrointestinal malignancies [J].

Chan, Annie On On ;

Rashid, Asif .

CURRENT MOLECULAR MEDICINE, 2006, 6 (04) :401-408

[5]

Single nucleotide polymorphism in DNMT3B promoter and its association with hepatocellular carcinoma in a Moroccan population [J].

Ezzikouri, Sayeh ;

Essaid El Feydi, Abdellah ;

Benazzouz, Mustapha ;

Afifi, Rajae ;

El Kihal, Latifa ;

Hassar, Mohammed ;

Akil, Abdellah ;

Pineau, Pascal ;

Benjelloun, Soumaya .

INFECTION GENETICS AND EVOLUTION, 2009, 9 (05) :877-881

[6]

Frank DA, 1999, MOL MED, V5, P432

[7]

Hepatitis C Virus Core Protein Triggers Hepatic Angiogenesis by a Mechanism Including Multiple Pathways [J].

Hassan, Mohamed ;

Selimovic, Denis ;

Ghozlan, Hanan ;

Abdel-kader, Ola .

HEPATOLOGY, 2009, 49 (05) :1469-1482

[8]

Hepatitis C Virus Infection Causes Cell Cycle Arrest at the Level of Initiation of Mitosis [J].

Kannan, Rathi P. ;

Hensley, Lucinda L. ;

Evers, Lauren E. ;

Lemon, Stanley M. ;

McGivern, David R. .

JOURNAL OF VIROLOGY, 2011, 85 (16) :7989-8001

[9]

Kong HJ, 2003, MOL CANCER RES, V1, P948

[10]

INITIATION OF REPLICATION OF THE HUMAN HEPATITIS-DELTA VIRUS GENOME FROM CLONED DNA - ROLE OF DELTA-ANTIGEN [J].

KUO, MYP ;

CHAO, M ;

TAYLOR, J .

JOURNAL OF VIROLOGY, 1989, 63 (05) :1945-1950

← 1 2 3 4 →