Single-synapse ablation and long-term imaging in live C. elegans

被引：53

作者：

Allen, Peter B. ^{[1
]}

Sgro, Allyson E. ^{[1
]}

Chao, Daniel L. ^{[2
]}

Doepker, Byron E. ^{[1
]}

Edgar, J. Scott ^{[1
]}

Shen, Kang ^{[2
]}

Chiu, Daniel T. ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

[2] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2008年 / 173卷 / 01期

关键词：

synapse; C; elegans; development; synaptogenesis; microfluidics; ablation; laser; LIF; imaging;

D O I：

10.1016/j.jneumeth.2008.05.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Synapses are individually operated, computational units for neural communication. To manipulate physically individual synapses in a living organism, we have developed a laser ablation technique for removing single synapses in live neurons in C. elegans that operates without apparent damage to the axon. As a complementary technique, we applied microfluidic immobilization of C. elegans to facilitate long-term fluorescence imaging and observation of neuronal development. With this technique, we directly demonstrated the existence of competition between developing synapses in the HSNL motor neuron. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：20 / 26

页数：7

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[2]

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[3]

Spatial regulation of an E3 ubiquitin ligase directs selective synapse elimination [J].