Antibodies against Echinococcus multilocularis alkaline phosphatase as markers for the specific diagnosis and the serological monitoring of alveolar echinococcosis

被引：26

作者：

Sarciron, EM ^{[1
]}

BressonHadni, S ^{[1
]}

Mercier, M ^{[1
]}

Lawton, P ^{[1
]}

Duranton, C ^{[1
]}

Lenys, D ^{[1
]}

Petavy, AF ^{[1
]}

Vuitton, DA ^{[1
]}

机构：

[1] WHO,COLLABORATING CTR PREVENT & TREATMENT HUMAN ECHIN,RES UNIT HLTH & RURAL ENVIRONM,BESANCON,FRANCE

来源：

PARASITE IMMUNOLOGY | 1997年 / 19卷 / 02期

关键词：

alveolar echinococcosis; alkaline phosphatase antibodies; specific diagnosis;

D O I：

10.1046/j.1365-3024.1997.d01-183.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immunological properties of the purified alkaline phosphatase (pAP) of Echinococcus multilocularis metacestodes have been investigated using alveolar echinococcosis (AE) patient sera in ELISA tests. A comparative study was done with EmC-Ag (crude antigen) and pAP-Ag (purified antigen). When the parasite purified enzyme pAP was used as antigen, the specificity of the ELISA was markedly increased since it reached 100% without any decrease of its sensitivity (100%). The serologic follow-up of AE patients was conducted during several months with these two antigens in three categories of patients: cured, stabilized and aggravated. There was a good correlation between clinical and serologic data when the pAP was used as antigen in ELISA tests, The anti-pAP antibodies titres did change more rapidly than anti-EmC antibodies titres when a recurrence occurred. Modifications of the anti-pAP antibodies levels were also observed during the patient's therapy: mebendazole, albendazole and Isoprinosine(R). These results suggest that pAP-Ag should be used for the diagnosis and the follow-up of AE patients.

引用

页码：61 / 68

页数：8

共 31 条

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