The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro

被引：80

作者：

DisqueKochem, C ^{[1
]}

Dreiseikelmann, B ^{[1
]}

机构：

[1] UNIV BIELEFELD,D-33501 BIELEFELD,GERMANY

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 19期

关键词：

D O I：

10.1128/jb.179.19.6133-6137.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The cytoplasmic protein TraM is one of four essential gene products of the F factor which are involved in DNA transfer after mating pair formation. TraM binds to three specific sites within the oriT region. Besides regulation of its own synthesis, the precise function of TraM during conjugation is not yet known. In the present work, the affinity of TraM to TraD was studied in vitro by an overlay assay and by affinity chromatography. Whether the interaction between TraM and TraD causes a transient or permanent anchoring of the F factor to the site of transfer is discussed, A 35-kDa host membrane protein of yet unknown function also shows affinity to TraM and may be involved in this anchoring process as well.

引用

页码：6133 / 6137

页数：5

共 27 条

[1] ENZYMIC UNWINDING OF DNA .2. CHAIN SEPARATION BY AN ATP-DEPENDENT DNA UNWINDING ENZYME [J].