A simple HPLC method for the simultaneous analysis of phosphatidylcholine and its partial hydrolysis products 1-and 2-acyl lysophosphatidylcholine

被引：27

作者：

Adlercreutz, D ^{[1
]}

Wehtje, E ^{[1
]}

机构：

[1] Lund Univ, Ctr Chem & Chem Engn, Dept Biotechnol, S-22100 Lund, Sweden

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 2001年 / 78卷 / 10期

基金：

瑞典研究理事会;

关键词：

acyl migration; enzymatic phospholipid modification; lysophosphatidylcholine; lysophospholipids; phosphatidylcholine; phospholipids;

D O I：

10.1007/s11746-001-0379-8

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A simple HPLC method for the simultaneous analysis of phosphatidylcholine (PC), 1-acyl lysophosphatidylcholine (1-acyl LPC), and 2-acyl lysophosphatidylcholine (2-acyl LPC) with refractive index detection is described. The separation of these three compounds was achieved on a Waters (Milford, MA) Spherisorb amino phase column using a mixture of ethanol and an aqueous oxalic acid solution as eluent. The optimal mixture of ethanol to oxalic acid solution was 92:8 (vol/vol). PC and the two regioisomers of LPC eluted within 15 min. The calibration curves were linear in a concentration range from 0.05 to 2.5 mM. Natural PC or LPC eluted in a single peak, despite the diversity in the fatty acid composition. There was no rearrangement between 1-acyl LPC and 2-acyl LPC during analysis or storage in ethanol within 23 h. This method is thus especially suitable for studying reactions on PC and acyl migration in LPC.

引用

页码：1007 / 1011

页数：5

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