Biologic and molecular characterization of Toxoplasma gondii isolates from pigs from Portugal

被引：64

作者：

de Sousa, S

Ajzenberg, D

Canada, N

Freire, L

da Costa, JMC

Dardé, ML

Thulliez, P

Dubey, JP ^{[1
]}

机构：

[1] USDA ARS, Anim Parasit Dis Lab, Anim & Nat Res Inst, BARC E, Beltsville, MD 20705 USA

[2] INSA, Ctr Parasite Immunol & Biol, P-4000509 Oporto, Portugal

[3] CHRU Dupuytren, Lab Parasitol Mycol, EA 3174, F-87042 Limoges, France

[4] Inst Biomed Sci Abel Salazar, P-4099003 Oporto, Portugal

[5] Inst Puericulture, Lab Toxoplasmose, F-75014 Paris, France

来源：

VETERINARY PARASITOLOGY | 2006年 / 135卷 / 02期

关键词：

Toxoplasma gondii; pigs; genotyping; Portugal; bioassay;

D O I：

10.1016/j.vetpar.2005.08.012

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Little is known of Toxoplasma gondii infections in animals in Portugal. In the present paper, we report the first isolation of viable T gondii from pigs in Portugal. Antibodies to T gondii were found in 52 (15.6%) of 333 pigs prior to slaughter using the modified agglutination test (MAT) at a serum dilution of 1:20. Attempts were made to isolate T gondii from 37 seropositive pigs. Samples of brain and/or heart from each pig were digested in acid pepsin, and bioassayed into mice. Viable T gondii was isolated from 15 pigs. Restriction fragment length polymorphism on products of SAG2 locus amplified by PCR and microsatellite analysis revealed that II isolates were Type 11 and four were Type III. The results indicate that phenotypically and genetically T gondii are similar to isolates from pigs from the U.S. Published by Elsevier B.V.

引用

页码：133 / 136

页数：4

共 18 条

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