Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy

被引：107

作者：

Preza, C

Snyder, DL

Conchello, JA

机构：

[1] Washington Univ, Inst Biomed Comp, St Louis, MO 63110 USA

[2] Washington Univ, Dept Elect Engn, St Louis, MO 63130 USA

来源：

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION | 1999年 / 16卷 / 09期

关键词：

D O I：

10.1364/JOSAA.16.002185

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Imaging models for differential-interference-contrast (DIC) microscopy are presented. Two- and three-dimensional models for DIC imaging under partially coherent illumination were derived and tested by using phantom specimens viewed with several conventional DIC microscopes and quasi-monochromatic light. DIC images recorded with a CCD camera were compared with model predictions that were generated by using theoretical point-spread functions, computer-generated phantoms, and estimated imaging parameters such as bias and sheer. Results show quantitative and qualitative agreement between model and data for several imaging conditions. (C) 1999 Optical Society of America [S0740-3232(99)01509-4] OCIS codes: 110.2990, 180.6900, 110.6880.

引用

页码：2185 / 2199

页数：15

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