Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria

被引:148
作者
Rusche, LN [1 ]
CruzReyes, J [1 ]
Piller, KJ [1 ]
SollnerWebb, B [1 ]
机构
[1] JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,BALTIMORE,MD 21205
关键词
editing complex; RNA editing; RNA lipase; Trypanosoma brucei; trypanosome;
D O I
10.1093/emboj/16.13.4069
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Kinetoplastid mitochondrial RNA editing, the insertion and deletion of U residues, is catalyzed by sequential cleavage, U addition or removal, and ligation reactions and is directed by complementary guide RNAs. We have purified a similar to 20S enzymatic complex from Trypanosoma brucei mitochondria that catalyzes a complete editing reaction in vitro, This complex possesses all four activities predicted to catalyze RNA editing: gRNA-directed endonuclease, terminal uridylyl transferase, 3' U-specific exonuclease, and RNA ligase, However, it does not contain other putative editing complex components: gRNA-independent endonuclease, RNA helicase, endogenous gRNAs or pre-mRNAs, or a 25 kDa gRNA-binding protein. The complex is composed of eight major polypeptides, three of which represent RNA ligase, These findings identify polypeptides representing catalytic editing factors, reveal the nature of this similar to 20S editing complex, and suggest a new model of editosome assembly.
引用
收藏
页码:4069 / 4081
页数:13
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