Intracellular accumulation of β-amyloid1-42 in neurons is facilitated by the α7 nicotinic acetylcholine receptor in Alzheimer's disease

被引:371
作者
Nagele, RG
D'Andrea, MR
Anderson, WJ
Wang, HY
机构
[1] Univ Med & Dent New Jersey, Sch Osteopath Med, Dept Mol Biol, Stratford, NJ 08084 USA
[2] RW Johnson Pharmaceut Res Inst, Spring House, PA 19477 USA
[3] CUNY, Sch Med, Dept Physiol & Pharmacol, New York, NY 10031 USA
关键词
A beta 42; nicotinic acetylcholine receptor; amyloid plaque; endocytosis; neuroblastoma; pyramidal cell;
D O I
10.1016/S0306-4522(01)00460-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Amyloid beta(1-42), a major component of amyloid plaques, binds with exceptionally high affinity to the alpha7 nicotinic acetylcholine receptor and accumulates intracellularly in neurons of Alzheimer's disease brains. In this study, we investigated the possibility that this binding plays a key role in facilitating intraneuronal accumulation of amyloid beta(1-42). Consecutive section immunohistochemistry and digital imaging were used to reveal the spatial relationship between amyloid beta(1-42) and the alpha7 receptor in affected neurons of Alzheimer's disease brains. Results showed that neurons containing substantial intracellular accumulations of amyloid beta(1-42) invariably express relatively high levels of the alpha7 receptor. Furthermore, this receptor is highly co-localized with amyloid beta(1-42) within neurons of Alzheimer's disease brains. To experimentally test the possibility that the binding interaction between exogenous amyloid beta(1-42) and the alpha7 receptor facilitates internalization and intracellular accumulation of amyloid beta(1-42) in Alzheimer's disease brains, we studied the fate of exogenous amyloid beta(1-42) and its interaction with the alpha7 receptor in vitro using cultured, transfected neuroblastoma cells that express elevated levels of this receptor. Transfected cells exhibited rapid binding, internalization and accumulation of exogenous amyloid beta(1-42), but not amyloid beta(1-40). Furthermore, the rate and extent of amyloid beta(1-42) internalization was related directly to the alpha7 receptor protein level, since (1) the rate of amyloid beta(1-42) accumulation was much lower in untransfected cells that express much lower levels of this receptor and (2) internalization was effectively blocked by alpha-bungarotoxin, an alpha7 receptor antagonist. As in neurons of Alzheimer's disease brains, the alpha7 receptor in transfected cells was precisely co-localized with amyloid beta(1-42) in prominent intracellular aggregates. Internalization of amyloid beta(1-42) in transfected cells was blocked by phenylarsine oxide, an inhibitor of endocytosis. We suggest that the intraneuronal accumulation of amyloid beta(1-42) in Alzheimer's disease brains occurs predominantly in neurons that express the alpha7 receptor. In addition, internalization of amyloid beta(1-42) may be facilitated by the high-affinity binding of amyloid beta(1-42) to the alpha7 receptor on neuronal cell surfaces, followed by endocytosis of the resulting complex. This provides a plausible explanation for the selective vulnerability of neurons expressing the alpha7 receptor in Alzheimer's disease brains and for the fact that amyloid beta(1-42) is the dominant amyloid beta peptide species in intracellular accumulations and amyloid plaques. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:199 / 211
页数:13
相关论文
共 77 条
[1]   Consensus recommendations for the postmortem diagnosis of Alzheimer's disease [J].
Ball, M ;
Braak, H ;
Coleman, P ;
Dickson, D ;
Duyckaerts, C ;
Gambetti, P ;
Hansen, L ;
Hyman, B ;
Jellinger, K ;
Markesbery, W ;
Perl, D ;
Powers, J ;
Price, J ;
Trojanowski, JQ ;
Wisniewski, H ;
Phelps, C ;
Khachaturian, Z .
NEUROBIOLOGY OF AGING, 1997, 18 (04) :S1-S2
[2]   Cellular expression of α7 nicotinic acetylcholine receptor protein in the temporal cortex in Alzheimer's and Parkinson's disease -: A stereological approach [J].
Banerjee, C ;
Nyengaard, JR ;
Wevers, A ;
de Vos, RAI ;
Steur, ENHJ ;
Lindstrom, J ;
Pilz, K ;
Nowacki, S ;
Bloch, W ;
Schröder, H .
NEUROBIOLOGY OF DISEASE, 2000, 7 (06) :666-672
[3]   Somatostatin-induced regulation of SST2A receptor expression and cell surface availability in central neurons:: Role of receptor internalization [J].
Boudin, H ;
Sarret, P ;
Mazella, J ;
Schonbrunn, A ;
Beaudet, A .
JOURNAL OF NEUROSCIENCE, 2000, 20 (16) :5932-5939
[4]   NEUROPATHOLOGICAL STAGING OF ALZHEIMER-RELATED CHANGES [J].
BRAAK, H ;
BRAAK, E .
ACTA NEUROPATHOLOGICA, 1991, 82 (04) :239-259
[5]  
Breese CR, 1997, J COMP NEUROL, V387, P385, DOI 10.1002/(SICI)1096-9861(19971027)387:3<385::AID-CNE5>3.0.CO
[6]  
2-X
[7]   Preferential adsorption, internalization and resistance to degradation of the major isoform of the Alzheimer's amyloid peptide, A beta 1-42, in differentiated PC12 cells [J].
Burdick, D ;
Kosmoski, J ;
Knauer, MF ;
Glabe, CG .
BRAIN RESEARCH, 1997, 746 (1-2) :275-284
[8]  
Burghaus L, 2000, MOL BRAIN RES, V76, P385
[9]   Neuron loss in APP transgenic mice [J].
Calhoun, ME ;
Wiederhold, KH ;
Abramowski, D ;
Phinney, AL ;
Probst, A ;
Sturchler-Pierrat, C ;
Staufenbiel, M ;
Sommer, B ;
Jucker, M .
NATURE, 1998, 395 (6704) :755-756
[10]   ENZYMATICALLY ACTIVE LYSOSOMAL PROTEASES ARE ASSOCIATED WITH AMYLOID DEPOSITS IN ALZHEIMER BRAIN [J].
CATALDO, AM ;
NIXON, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (10) :3861-3865