Abnormal structure of the Y chromosome detected in bovine gonadal hypoplasia (XY female) by FISH

被引：19

作者：

Kawakura, K

Miyake, YI

Murakami, RK

Kondoh, S

Hirata, TI

Kaneda, Y

机构：

[1] IWATE UNIV,FAC AGR,DEPT VET MED,LAB THERIOGENOL,MORIOKA,IWATE 020,JAPAN

[2] NATL INST ANIM IND,TSUKUBA,IBARAKI,JAPAN

[3] MORIOKA LIVESTOCK HYG SERV CTR,TAKIZAWA,JAPAN

[4] HOKKAIDO PREFECTURE FEDERAT AGR MUTUAL AID ASSOC,SARUFUTSU,JAPAN

[5] IWATE UNIV,FAC AGR,EDUC FARM,SHIZUKUISHI,JAPAN

[6] TOKYO UNIV AGR & TECHNOL,FAC AGR,DEPT VET MED,LAB THERIOGENOL,FUCHU,TOKYO 183,JAPAN

来源：

CYTOGENETICS AND CELL GENETICS | 1997年 / 76卷 / 1-2期

关键词：

D O I：

10.1159/000134508

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The structure of the Y chromosome was investigated by FISH and G banding in 3 cases of bovine XY females shown by PCR to lack the SRY gene. Although 2 different repeat-DNA sequences (BC1.2 and btDYZ-1) hybridized to the short arm of the Y chromosome in a normal bull, they hybridized to both arms of the Y chromosome in the XY females. In the Y chromosome from XY females, only the centromeric region was darkly stained by G banding, in comparison with dark staining on the long arm of a normal Y chromosome. From the results obtained, it seemed that the Y chromosome from the XY females was structurally abnormal, namely an isochromosome with an identical short arm. Our results also confirm that SRY is located on the long arm of the Y chromosome in bovine.

引用

页码：36 / 38

页数：3

共 22 条

[1] MAPPING THE TESTIS DETERMINANTS BY AN ANALYSIS OF Y-SPECIFIC SEQUENCES IN MALES WITH APPARENT XX AND XO KARYOTYPES AND FEMALES WITH XY KARYOTYPES [J].