Effect of eluent on the ionization efficiency of flavonoids by ion spray, atmospheric pressure chemical ionization, and atmospheric pressure photoionization mass spectrometry

被引:138
作者
Rauha, JP
Vuorela, H
Kostiainen, R
机构
[1] Univ Helsinki, Dept Pharm, Div Pharmaceut Chem, FIN-00014 Helsinki, Finland
[2] Univ Helsinki, Dept Pharm, Div Pharmaceut Chem, FIN-00014 Helsinki, Finland
[3] Univ Helsinki, Dept Pharm, Viikki Drug Discovery Technol Ctr DDTC, FIN-00014 Helsinki, Finland
来源
JOURNAL OF MASS SPECTROMETRY | 2001年 / 36卷 / 12期
关键词
flavonoids; ion spray; APCI; APPI; buffer;
D O I
10.1002/jms.231
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The effect of nine different eluent compositions on the ionization efficiency of five flavonoids was studied using ion spray (IS), atmospheric pressure chemical ionization (APCI), and the novel atmospheric pressure photoionization (APPI), in positive and negative ion modes. The eluent composition had a great effect on the ionization efficiency, and the optimal ionization conditions were achieved in positive ion IS and APCI using 0.4% formic acid (pH 2.3) as a buffer, and in negative ion IS and APCI using ammonium acetate buffer adjusted to pH 4.0. For APPI work, the eluent of choice appeared to be a mixture of organic solvent and 5 mm aqueous ammonium acetate. The limits of detection (LODs) were determined in scan mode for the analytes by liquid chromatography/mass spectrometry using IS, APCI and APPI interfaces. The results show that negative ion IS with an eluent system consisting of acidic ammonium acetate buffer provides the best conditions for detection of flavonoids in mass spectrometry mode, their LODs being between 0.8 and 13 mum for an injection volume of 20 mul. Copyright (C) 2001 John Wiley Sons, Ltd.
引用
收藏
页码:1269 / 1280
页数:12
相关论文
共 55 条
[1]   Acid/base properties of flavonoids hydroxylated at positions 2 and 3:: a novel quantum mechanical study in gas-phase and solution [J].
Alemán, C .
JOURNAL OF MOLECULAR STRUCTURE-THEOCHEM, 2000, 528 :65-73
[2]   Determination of selected phytochemicals by reversed-phase high-performance liquid chromatography combined with ultraviolet and mass spectrometric detection [J].
Andlauer, W ;
Martena, MJ ;
Fürst, P .
JOURNAL OF CHROMATOGRAPHY A, 1999, 849 (02) :341-348
[3]   FAST ATOM BOMBARDMENT AND FAST ATOM BOMBARDMENT COLLISION-ACTIVATED DISSOCIATION MASS-ANALYZED ION KINETIC-ENERGY ANALYSIS OF C-GLYCOSIDIC FLAVONOIDS [J].
BECCHI, M ;
FRAISSE, D .
BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1989, 18 (02) :122-130
[4]   GAS-PHASE ACIDITIES OF ALIPHATIC CARBOXYLIC-ACIDS, BASED ON MEASUREMENTS OF PROTON-TRANSFER EQUILIBRIA [J].
CALDWELL, G ;
RENNEBOOG, R ;
KEBARLE, P .
CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1989, 67 (04) :611-618
[5]  
Careri M, 1999, RAPID COMMUN MASS SP, V13, P2399, DOI 10.1002/(SICI)1097-0231(19991215)13:23<2399::AID-RCM805>3.0.CO
[6]  
2-N
[7]  
COVEY TR, 1986, ANAL CHEM, V58, P1451
[8]   CAPILLARY COLUMN GAS-CHROMATOGRAPHY OF METHYL AND TRIMETHYLSILYL DERIVATIVES OF SOME NATURALLY-OCCURRING FLAVONOID AGLYCONES AND OTHER PHENOLICS [J].
CREASER, CS ;
KOUPAIABYAZANI, MR ;
STEPHENSON, GR .
JOURNAL OF CHROMATOGRAPHY, 1989, 478 (02) :415-421
[9]   FAST-ATOM-BOMBARDMENT AND TANDEM MASS-SPECTROMETRY FOR STRUCTURE DETERMINATION OF STEROID AND FLAVONOID GLYCOSIDES [J].
CROW, FW ;
TOMER, KB ;
LOOKER, JH ;
GROSS, ML .
ANALYTICAL BIOCHEMISTRY, 1986, 155 (02) :286-307
[10]  
da Costa CT, 2000, J MASS SPECTROM, V35, P540, DOI 10.1002/(SICI)1096-9888(200004)35:4<540::AID-JMS966>3.0.CO