Epitope tagging genomic DNA using a CD-tagging Tn10 minitransposon

被引：15

作者：

Telmer, CA ^{[1
]}

Berget, PB ^{[1
]}

Ballou, B ^{[1
]}

Murphy, RF ^{[1
]}

Jarvik, JW ^{[1
]}

机构：

[1] Carnegie Mellon Univ, Dept Sci Biol, Pittsburgh, PA 15213 USA

来源：

BIOTECHNIQUES | 2002年 / 32卷 / 02期

关键词：

D O I：

10.2144/02322rr04

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we describe an efficient system for epitope tagging cloned genes by CD tagging using a mini-Tn10 transposon delivery vector The system was tested against a lambdaFIX genomic clone of the human nucleolin gene. Transfection of HeLa cells with the tagged gene led to the expression of both the appropriately spliced tagged transcript and the appropriately localized tagged protein.

引用

页码：422 / +

页数：7

共 14 条

[1]

Bachmann BJ, 1987, ESCHERICHIA COLI SAL, V2, P1190

[2] CHARACTERIZATION OF TN10D-CAM - A TRANSPOSITION-DEFECTIVE TN10 SPECIFYING CHLORAMPHENICOL RESISTANCE [J].

ELLIOTT, T ;

ROTH, JR .

MOLECULAR AND GENERAL GENETICS, 1988, 213 (2-3) :332-338

[3]

FARKAS DL, 1993, ANNU REV PHYSIOL, V55, P785, DOI 10.1146/annurev.ph.55.030193.004033

[4]

Ginisty H, 1999, J CELL SCI, V112, P761

[5]

GRANA D, 1988, GENETICS, V120, P319

[6] NOTI GENOMIC CLEAVAGE MAP OF ESCHERICHIA-COLI K-12 STRAIN MG1655 [J].

HEATH, JD ;

PERKINS, JD ;

SHARMA, B ;

WEINSTOCK, GM .

JOURNAL OF BACTERIOLOGY, 1992, 174 (02) :558-567

[7]

Jarvik JW, 1996, BIOTECHNIQUES, V20, P896

[8] Epitope tagging [J].

Jarvik, JW ;

Telmer, CA .

ANNUAL REVIEW OF GENETICS, 1998, 32 :601-618

[9]

KLECKNER N, 1991, METHOD ENZYMOL, V204, P139

[10] LAMBDOID PHAGES THAT SIMPLIFY RECOVERY OF INVITRO RECOMBINANTS [J].

MURRAY, NE ;

BRAMMAR, WJ ;

MURRAY, K .

MOLECULAR & GENERAL GENETICS, 1977, 150 (01) :53-61

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