Requirement of a corepressor for Dr1-mediated repression of transcription

被引：116

作者：

Mermelstein, F

Yeung, K

Cao, J

Inostroza, JA

ErdjumentBromage, H

Eagelson, K

Landsman, D

Levitt, P

Tempst, P

Reinberg, D

机构：

[1] UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, DEPT BIOCHEM, HOWARD HUGHES MED INST, PISCATAWAY, NJ 08854 USA

[2] MEM SLOAN KETTERING CANC CTR, PROGRAM MOLEC BIOL, NEW YORK, NY 10021 USA

[3] UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, DEPT NEUROBIOL, PISCATAWAY, NJ 08854 USA

[4] NATL LIB MED, NATL CTR BIOTECHNOL INFORMAT, NIH, BETHESDA, MD 20894 USA

来源：

GENES & DEVELOPMENT | 1996年 / 10卷 / 08期

关键词：

Dr1-mediated repression; corepression of transcription; HeLa cells; heterodimer formation;

D O I：

10.1101/gad.10.8.1033

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A Dr1-associated polypeptide (DRAP1) was isolated from HeLa cells and found to function as a corepressor of transcription. Corepressor function requires an interaction between DRAP1 and Dr1. Heterodimer formation was dependent on a histone fold moth present at the amino terminus of both polypeptides. Association of DRAP1 with Dr1 results in higher stability of the Dr1-TBP-TATA moth complex and precluded the entry of TPIIA and/or TFIIB to preinitiation complexes. DRAP1 was found to be expressed in all tissues analyzed with higher levels in tissues with a low mitotic index. Analysis of DRAP1 in the developing brain of rat demonstrated undetectable levels of DRAP1 in actively dividing cells but high levels of DRAP1 expression in differentiated non dividing cells. Dr1 was immunodetected in all cells analyzed. A model for DRAP1-dependent, Dr1-mediated repression of transcription is proposed.

引用

页码：1033 / 1048

页数：16

共 82 条

[1] THE PSEUDORABIES IMMEDIATE EARLY PROTEIN STIMULATES INVITRO TRANSCRIPTION BY FACILITATING TFIID - PROMOTER INTERACTIONS [J].

ABMAYR, SM ;

WORKMAN, JL ;

ROEDER, RG .

GENES & DEVELOPMENT, 1988, 2 (05) :542-553

[2]

[Anonymous], 1970, Anat Rec, V166, P257

[3]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[4] AN ATP-DEPENDENT INHIBITOR OF TBP BINDING TO DNA [J].

AUBLE, DT ;

HAHN, S .

GENES & DEVELOPMENT, 1993, 7 (05) :844-856

[5] MAD-MAX TRANSCRIPTIONAL REPRESSION IS MEDIATED BY TERNARY COMPLEX-FORMATION WITH MAMMALIAN HOMOLOGS OF YEAST REPRESSOR SIN3 [J].

AYER, DE ;

LAWRENCE, QA ;

EISENMAN, RN .

CELL, 1995, 80 (05) :767-776

[6] INTERACTION OF HUMAN THYROID-HORMONE RECEPTOR-BETA WITH TRANSCRIPTION FACTOR TFIIB MAY MEDIATE TARGET GENE DEREPRESSION AND ACTIVATION BY THYROID-HORMONE [J].

BANIAHMAD, A ;

HA, I ;

REINBERG, D ;

TSAI, S ;

TSAI, MJ ;

OMALLEY, BW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (19) :8832-8836

[7] A VARIETY OF DNA-BINDING AND MULTIMERIC PROTEINS CONTAIN THE HISTONE FOLD MOTIF [J].

BAXEVANIS, AD ;

ARENTS, G ;

MOUDRIANAKIS, EN ;

LANDSMAN, D .

NUCLEIC ACIDS RESEARCH, 1995, 23 (14) :2685-2691

[8] 5 INTERMEDIATE COMPLEXES IN TRANSCRIPTION INITIATION BY RNA POLYMERASE-II [J].

BURATOWSKI, S ;

HAHN, S ;

GUARENTE, L ;

SHARP, PA .

CELL, 1989, 56 (04) :549-561

[9] THE PRIMARY STRUCTURE OF RAT RIBOSOMAL PROTEIN-S9 [J].

CHAN, YL ;

PAZ, V ;

OLVERA, J ;

WOOL, IG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 193 (01) :106-112

[10] A TRANSCRIPTIONAL CO-REPRESSOR THAT INTERACTS WITH NUCLEAR HORMONE RECEPTORS [J].

CHEN, JD ;

EVANS, RM .

NATURE, 1995, 377 (6548) :454-457

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