Subtilosin production by two Bacillus subtilis subspecies and variance of the sbo-alb cluster

被引：71

作者：

Stein, T ^{[1
]}

Düsterhus, S ^{[1
]}

Stroh, A ^{[1
]}

Entian, KD ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, Inst Mikrobiol, D-60439 Frankfurt, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 04期

关键词：

D O I：

10.1128/AEM.70.4.2349-2353.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Eight different Bacillus subtilis strains and Bacillus atrophaeus were found to produce the bacteriocin subtilosin A. On the basis of the subtilosin gene (sbo) sequences two distinct classes of B. subtilis strains were distinguished, and they fell into the two B. subtilis subspecies (B. subtilis subsp. subtilis and B. subtilis subsp. spizizenii). The entire sequence of the subtilosin gene cluster of a B. subtilis subsp. spizizenii strain, B. subtilis ATCC 6633, was determined. This sequence exhibited a high level of homology to the sequence of the sbo-alb gene locus of B. subtilis 168. By using primer extension analysis the transcriptional start sites of sbo in B. subtilis strains ATCC 6633 and 168 were found to be 47 and 45 bp upstream of the sbo start codon, respectively. Our results provide insight into the incipient evolutionary divergence of the two B. subtilis subspecies.

引用

页码：2349 / 2353

页数：5

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