A nanotube based electron microbeam cellular irradiator for radiobiology research

被引：8

作者：

Bordelon, David E. ^{[2
]}

Zhang, Jian ^{[1
]}

Graboski, Sarah ^{[1
]}

Cox, Adrienne ^{[1
]}

Schreiber, Eric ^{[1
]}

Zhou, Otto Z. ^{[3
,4
]}

Chang, Sha ^{[1
]}

机构：

[1] Univ N Carolina, Dept Radiat Oncol, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Curriculum Appl & Mat Sci, Chapel Hill, NC 27599 USA

[3] Univ N Carolina, Curriculum Appl & Mat Sci, Dept Phys & Astron, Chapel Hill, NC 27599 USA

[4] Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2008年 / 79卷 / 12期

关键词：

biomolecular effects of radiation; cancer; carbon nanotubes; cellular effects of radiation; DNA; dosimetry; nanobiotechnology; radiation therapy; tumours;

D O I：

10.1063/1.3043417

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 [仪器科学与技术]; 080401 [精密仪器及机械]; 081102 [检测技术与自动化装置];

摘要：

A prototype cellular irradiator utilizing a carbon nanotube (CNT) based field emission electron source has been developed for microscopic image-guided cellular region irradiation. The CNT cellular irradiation system has shown great potential to be a high temporal and spatial resolution research tool to enable researchers to gain a better understanding of the intricate cellular and intercellular microprocesses occurring following radiation deposition, which is essential to improving radiotherapy cancer treatment outcomes. In this paper, initial results of the system development are reported. The relationship between field emission current, the dose rate, and the dose distribution has been investigated. A beam size of 23 mu m has been achieved with variable dose rates of 1-100 Gy/s, and the system dosimetry has been measured using a radiochromic film. Cell irradiation has been demonstrated by the visualization of H2AX phosphorylation at DNA double-strand break sites following irradiation in a rat fibroblast cell monolayer. The prototype single beam cellular irradiator is a preliminary step to a multipixel cell irradiator that is under development.

引用

页数：5

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