The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains

被引：110

作者：

Brueggeman, R. ^{[1
]}

Druka, A. ^{[2
]}

Nirmala, J. ^{[1
]}

Cavileer, T. ^{[3
]}

Drader, T. ^{[4
]}

Rostoks, N. ^{[5
]}

Mirlohi, A. ^{[6
]}

Bennypaul, H. ^{[7
]}

Gill, U. ^{[1
]}

Kudrna, D. ^{[8
]}

Whitelaw, C. ^{[9
,10
]}

Kilian, A. ^{[11
]}

Han, F. ^{[12
]}

Sun, Y. ^{[13
]}

Gill, K. ^{[1
]}

Steffenson, B. ^{[14
]}

Kleinhofs, A. ^{[1
,4
]}

机构：

[1] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA

[2] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland

[3] Univ Idaho, Dept Biol Sci, Moscow, ID 83844 USA

[4] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA

[5] Latvian State Univ, Fac Biol, LV-1586 Riga, Latvia

[6] Isfahan Univ Technol, Coll Agr, Esfahan, Iran

[7] CFIA Charlottetown Lab, Charlottetown, PE C1A 5T1, Canada

[8] Univ Arizona, Arizona Genom Inst, Dept Plant Sci, Tucson, AZ 85721 USA

[9] Natl Univ Ireland, Res Off, Maynooth, Kildare, Ireland

[10] Natl Univ Ireland, Grad Studies, Maynooth, Kildare, Ireland

[11] CSIRO Forestry 1 Wilf Crane Crescent, Divers Arrays Technol Pty Ltd, Yarralumla, ACT 2600, Australia

[12] Pioneer HiBred Int Inc, Johnston, IA 50131 USA

[13] Monsanto Co, Waterman, IL 60556 USA

[14] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 39期

关键词：

actin depolymerizing factor; barley; disease resistance domains; map-based cloning;

D O I：

10.1073/pnas.0807270105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We isolated the barley stem rust resistance genes Rpg5 and rpg4 by map-based cloning. These genes are colocalized on a 70-kb genomic region that was delimited by recombination. The Rpg5 gene consists of an unusual structure encoding three typical plant disease resistance protein domains: nucleotide-binding site, leucine-rich repeat, and serine threonine protein kinase. The predicted RPG5 protein has two putative transmembrane sites possibly involved in membrane binding. The gene is expressed at low but detectable levels. Posttranscriptional gene silencing using VIGS resulted in a compatible reaction with a normally incompatible stem rust pathogen. Allele sequencing also validated the candidate Rpg5 gene. Allele and recombinant sequencing suggested that the probable rpg4 gene encoded an actin depolymerizing factor-like protein. Involvement of actin depolymerizing factor genes in nonhost resistance has been documented, but discovery of their role in gene-for-gene interaction would be novel and needs to be further substantiated.

引用

页码：14970 / 14975

页数：6

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