Giant eyes in Xenopus laevis by overexpression of XOptx2

被引:165
作者
Zuber, ME
Perron, M
Philpott, A
Bang, A
Harris, WA
机构
[1] Univ Cambridge, Dept Anat, Cambridge CB2 3DY, England
[2] Univ Cambridge, Dept Oncol, Cambridge CB3 2QQ, England
[3] Salk Inst Biol Studies, Mol Neurobiol Lab, San Diego, CA 92186 USA
基金
英国惠康基金;
关键词
D O I
10.1016/S0092-8674(00)81963-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Overexpression of XOptx2, a homeodomain-containing transcription factor expressed in the Xenopus embryonic eye field, results in a dramatic increase in eye size. An XOptx2-Engrailed repressor gives a similar phenotype, while an XOptx2-VP16 activator reduces eye size. XOptx2 stimulates bromodeoxyuridine incorporation, and XOptx2-induced eye enlargement is dependent on cellular proliferation. Moreover, retinoblasts transfected with XOptx2 produce clones of cells approximately twice as large as control clones. Pax6, which does not increase eye size alone, acts synergistically with XOptx2. Our results suggest that XOptx2, in combination with other genes expressed in the eye field, is crucially involved in the proliferative state of retinoblasts and thereby the size of the eye.
引用
收藏
页码:341 / 352
页数:12
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