Epithelial expression of interleukin-37b in inflammatory bowel disease

被引：181

作者：

Imaeda, H. ^{[1
]}

Takahashi, K. ^{[3
]}

Fujimoto, T. ^{[3
]}

Kasumi, E. ^{[1
]}

Ban, H. ^{[1
]}

Bamba, S. ^{[1
]}

Sonoda, H. ^{[2
]}

Shimizu, T. ^{[2
]}

Fujiyama, Y. ^{[1
]}

Andoh, A. ^{[3
]}

机构：

[1] Shiga Univ Med Sci, Dept Med, Otsu, Shiga 5202192, Japan

[2] Shiga Univ Med Sci, Dept Surg, Otsu, Shiga 5202192, Japan

[3] Shiga Univ Med Sci, Grad Sch, Div Mucosal Immunol, Otsu, Shiga 5202192, Japan

来源：

CLINICAL AND EXPERIMENTAL IMMUNOLOGY | 2013年 / 172卷 / 03期

关键词：

anti-inflammatory cytokine; innate immunity; TNF-alpha; COLONIC SUBEPITHELIAL MYOFIBROBLASTS; PROTEIN-KINASE KINASE; IL-1; FAMILY; CROHNS-DISEASE; KAPPA-B; INHIBITOR; CELL; OVEREXPRESSION; IDENTIFICATION; PATHOGENESIS;

D O I：

10.1111/cei.12061

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Interleukin (IL)-37 is a member of the IL-1 cytokine family. We investigated IL-37b expression in the inflamed mucosa of inflammatory bowel disease (IBD) patients. Furthermore, we analysed IL-37b expression in human colonic epithelial cells. The human colonic epithelial cell line T84 and human colonic subepithelial myofibroblasts (SEMFs) were used. IL-37b expression in the IBD mucosa was evaluated by immunohistochemistry. IL-37b mRNA and protein expression were determined by real time-polymerase chain reaction (PCR) and Western blotting, respectively. IL-37b was not detected in the normal colonic mucosa. In the inflamed mucosa of IBD patients, epithelial IL-37b expression was increased markedly. In ulcerative colitis (UC) and Crohn's disease (CD) patients, IL-37b expression was enhanced in the affected mucosa. In the intestinal epithelial cell line T84, the expression of IL-37b mRNA and protein was enhanced by tumour necrosis factor (TNF)-. This IL-37b induction by TNF- was mediated by nuclear factor (NF)-B and activator protein (AP)-1 activation. Furthermore, IL-37b inhibited TNF--induced interferon--inducible protein (IP)-10 expression significantly in human colonic SEMFs. Epithelial IL-37b expression was increased in IBD patients, especially UC patients. IL-37b may be involved in the pathophysiology of IBD as an anti-inflammatory cytokine and an inhibitor of both innate and acquired immune responses.

引用

页码：410 / 416

页数：7

共 33 条

[1]

PD-098059 IS A SPECIFIC INHIBITOR OF THE ACTIVATION OF MITOGEN-ACTIVATED PROTEIN-KINASE KINASE IN-VITRO AND IN-VIVO [J].