Epithelial expression of interleukin-37b in inflammatory bowel disease

被引:181
作者
Imaeda, H. [1 ]
Takahashi, K. [3 ]
Fujimoto, T. [3 ]
Kasumi, E. [1 ]
Ban, H. [1 ]
Bamba, S. [1 ]
Sonoda, H. [2 ]
Shimizu, T. [2 ]
Fujiyama, Y. [1 ]
Andoh, A. [3 ]
机构
[1] Shiga Univ Med Sci, Dept Med, Otsu, Shiga 5202192, Japan
[2] Shiga Univ Med Sci, Dept Surg, Otsu, Shiga 5202192, Japan
[3] Shiga Univ Med Sci, Grad Sch, Div Mucosal Immunol, Otsu, Shiga 5202192, Japan
关键词
anti-inflammatory cytokine; innate immunity; TNF-alpha; COLONIC SUBEPITHELIAL MYOFIBROBLASTS; PROTEIN-KINASE KINASE; IL-1; FAMILY; CROHNS-DISEASE; KAPPA-B; INHIBITOR; CELL; OVEREXPRESSION; IDENTIFICATION; PATHOGENESIS;
D O I
10.1111/cei.12061
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
071005 [微生物学]; 100108 [医学免疫学];
摘要
Interleukin (IL)-37 is a member of the IL-1 cytokine family. We investigated IL-37b expression in the inflamed mucosa of inflammatory bowel disease (IBD) patients. Furthermore, we analysed IL-37b expression in human colonic epithelial cells. The human colonic epithelial cell line T84 and human colonic subepithelial myofibroblasts (SEMFs) were used. IL-37b expression in the IBD mucosa was evaluated by immunohistochemistry. IL-37b mRNA and protein expression were determined by real time-polymerase chain reaction (PCR) and Western blotting, respectively. IL-37b was not detected in the normal colonic mucosa. In the inflamed mucosa of IBD patients, epithelial IL-37b expression was increased markedly. In ulcerative colitis (UC) and Crohn's disease (CD) patients, IL-37b expression was enhanced in the affected mucosa. In the intestinal epithelial cell line T84, the expression of IL-37b mRNA and protein was enhanced by tumour necrosis factor (TNF)-. This IL-37b induction by TNF- was mediated by nuclear factor (NF)-B and activator protein (AP)-1 activation. Furthermore, IL-37b inhibited TNF--induced interferon--inducible protein (IP)-10 expression significantly in human colonic SEMFs. Epithelial IL-37b expression was increased in IBD patients, especially UC patients. IL-37b may be involved in the pathophysiology of IBD as an anti-inflammatory cytokine and an inhibitor of both innate and acquired immune responses.
引用
收藏
页码:410 / 416
页数:7
相关论文
共 33 条
[1]
PD-098059 IS A SPECIFIC INHIBITOR OF THE ACTIVATION OF MITOGEN-ACTIVATED PROTEIN-KINASE KINASE IN-VITRO AND IN-VIVO [J].
ALESSI, DR ;
CUENDA, A ;
COHEN, P ;
DUDLEY, DT ;
SALTIEL, AR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (46) :27489-27494
[2]
IL-17 selectively down-regulates TNF-α-induced RANTES gene expression in human colonic subepithelial myofibroblasts [J].
Andoh, A ;
Fujino, S ;
Bamba, S ;
Araki, Y ;
Okuno, T ;
Bamba, T ;
Fujiyama, Y .
JOURNAL OF IMMUNOLOGY, 2002, 169 (04) :1683-1687
[3]
Multicenter analysis of fecal microbiota profiles in Japanese patients with Crohn's disease [J].
Andoh, Akira ;
Kuzuoka, Hiroyuki ;
Tsujikawa, Tomoyuki ;
Nakamura, Shiro ;
Hirai, Fumihito ;
Suzuki, Yasuo ;
Matsui, Toshiyuki ;
Fujiyama, Yoshihide ;
Matsumoto, Takayuki .
JOURNAL OF GASTROENTEROLOGY, 2012, 47 (12) :1298-1307
[4]
Comparison of the fecal microbiota profiles between ulcerative colitis and Crohn's disease using terminal restriction fragment length polymorphism analysis [J].
Andoh, Akira ;
Imaeda, Hirotsugu ;
Aomatsu, Tomoki ;
Inatomi, Osamu ;
Bamba, Shigeki ;
Sasaki, Masaya ;
Saito, Yasuharu ;
Tsujikawa, Tomoyuki ;
Fujiyama, Yoshihide .
JOURNAL OF GASTROENTEROLOGY, 2011, 46 (04) :479-486
[5]
BEST WR, 1979, GASTROENTEROLOGY, V77, P843
[6]
IL-37: a new anti-inflammatory cytokine of the IL-1 family [J].
Boraschi, Diana ;
Lucchesi, Davide ;
Hainzl, Stefan ;
Leitner, Maria ;
Maier, Elisabeth ;
Mangelberger, Doris ;
Oostingh, Gertie J. ;
Pfaller, Tobias ;
Pixner, Claudia ;
Posselt, Gernot ;
Italiani, Paola ;
Nold, Marcel F. ;
Nold-Petry, Claudia A. ;
Bufler, Philip ;
Dinarello, Charles A. .
EUROPEAN CYTOKINE NETWORK, 2011, 22 (03) :127-147
[7]
Interleukin-1 homologues lL-1F7b and IL-18 contain functional mRNA instability elements within the coding region responsive to lipopolysaccharide [J].
Bufler, P ;
Gamboni-Robertson, F ;
Azam, T ;
Kim, SH ;
Dinarello, CA .
BIOCHEMICAL JOURNAL, 2004, 381 :503-510
[8]
A complex of the IL-1 homologue IL-1F7b and IL-18-binding protein reduces IL-18 activity [J].
Bufler, P ;
Azam, T ;
Gamboni-Robertson, F ;
Reznikov, LL ;
Kumar, S ;
Dinarello, CA ;
Kim, SH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (21) :13723-13728
[9]
SB-203580 IS A SPECIFIC INHIBITOR OF A MAP KINASE HOMOLOG WHICH IS STIMULATED BY CELLULAR STRESSES AND INTERLEUKIN-1 [J].
CUENDA, A ;
ROUSE, J ;
DOZA, YN ;
MEIER, R ;
COHEN, P ;
GALLAGHER, TF ;
YOUNG, PR ;
LEE, JC .
FEBS LETTERS, 1995, 364 (02) :229-233
[10]
A HUMAN COLONIC TUMOR-CELL LINE THAT MAINTAINS VECTORIAL ELECTROLYTE TRANSPORT [J].
DHARMSATHAPHORN, K ;
MCROBERTS, JA ;
MANDEL, KG ;
TISDALE, LD ;
MASUI, H .
AMERICAN JOURNAL OF PHYSIOLOGY, 1984, 246 (02) :G204-G208