In vitro study of bacterial degradation of ethyl glucuronide and ethyl sulphate

被引：80

作者：

Baranowski, Stefanie ^{[1
]}

Serr, Annerose ^{[2
]}

Thierauf, Annette ^{[1
]}

Weinmann, Wolfgang ^{[1
]}

Perdekamp, Markus Gross ^{[1
]}

Wurst, Friedrich M. ^{[3
]}

Halter, Claudia C. ^{[1
]}

机构：

[1] Univ Freiburg, Med Ctr, Inst Forens Med, D-79104 Freiburg, Germany

[2] Univ Freiburg, Med Ctr, Inst Med Microbiol & Hyg, Dept Microbiol & Hyg, D-79104 Freiburg, Germany

[3] Paracelsus Med Univ, Christian Doppler Clin, A-5020 Salzburg, Austria

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 2008年 / 122卷 / 05期

关键词：

bacterial degradation; glucuronidase; ethyl sulphate; ethyl glucuronide;

D O I：

10.1007/s00414-008-0229-3

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 [法学]; 10 [医学];

摘要：

Recent studies show that ethyl glucuronide (EtG) can be decomposed by bacteria; whilst so far no degradation of ethyl sulphate (EtS) has been observed. In the present study, in vitro experiments with bacterial colonies were performed. Bacteria (Escherichia coli, Klebsiella pneumoniae, Clostridium sordellii) were isolated from autopsy material (liver, heart blood, urine, ascites, pericardial fluid, pleural fluid) tested for beta-glucuronidase activity, and three bacterial strains were added to nutrient-deficient medium ontaining EtG and/or EtS and incubated at 36 +/- 1 degrees C. Samples were taken after various intervals up to 11 days, and EtG and EtS were determined by electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS). EtG was degraded by E. coli and C. sordellii-complete degradation occurred in the range of 3-4 days-and these bacteria exhibited beta-glucuronidase activity. EtS was not affected within 11 days of incubation.

引用

页码：389 / 393

页数：5

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