Recovery of a neurovirulent human coronavirus OC43 from an infectious cDNA clone

被引：72

作者：

St-Jean, JR

Desforges, M

Almazán, F

Jacomy, H

Enjuanes, L

Talbot, PJ

机构：

[1] INRS Energie, Inst Armand Frappier, Lab Neuroimmunovirol, Laval, PQ H7V 1B7, Canada

[2] CSIC, Dept Mol & Cell Biol, Ctr Nacl Biotecnol, E-28049 Madrid, Spain

来源：

JOURNAL OF VIROLOGY | 2006年 / 80卷 / 07期

关键词：

D O I：

10.1128/JVI.80.7.3670-3674.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study describes the assembly of a full-length cDNA clone of human coronavirus (HCoV)-OC43 in a bacterial artificial chromosome (BAC). The BAC containing the full-length infectious cDNA (pBAC-OC43(FL)) was assembled using a two-part strategy. The first step consisted in the introduction of each end of the viral genome into the BAC with accessory sequences allowing proper transcription. The second step consisted in the insertion of the whole HCoV-OC43 cDNA genome into the BAC. To produce recombinant viral particles, pBAC-OC43(FL) was transfected into BHK-21 cells. Recombinant virus displayed the same phenotypic properties as the wild-type virus, including infectious virus titers produced in cell culture and neurovirulence in mice.

引用

收藏

页码：3670 / 3674

页数：5

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