Acute effects of alcohol on larval zebrafish: a genetic system for large-scale screening

被引:169
作者
Lockwood, B
Bjerke, S
Kobayashi, K
Guo, S [1 ]
机构
[1] Univ Calif San Francisco, Wheeler Ctr Neurobiol Addict, Dept Biopharmaceut Sci, Program Human Genet, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Wheeler Ctr Neurobiol Addict, Dept Biopharmaceut Sci, Program Biol Sci, San Francisco, CA 94143 USA
关键词
alcohol effects; behavior; drugs of abuse; genetics; larval zebrafish; locomotor activity;
D O I
10.1016/j.pbb.2004.01.003
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
Larval zebrafish are used extensively for developmental genetic studies due to their salient features, such as small size, external development, optical transparency, and accessibility in large numbers. However, their use for the study of drug and alcohol abuse has not been explored. Here we investigated the response of larval zebrafish to acute treatment of alcohol. Our analyses showed that like adults, the larval zebrafish exhibited a dose-dependent locomotor response to ethanol: intermediate doses led to hyperactivity, whereas high doses have a neurodepressive effect resulting in hypoactivity and sedation. Alcohol also induced morphological changes of melanocytes, providing a visible cellular measure of the biological effects of alcohol in vivo. In addition, alcohol induced thigmotaxis behavior (preference for the edge of a compartment). In the behaviors we analyzed, genetic background influenced the locomotor responses to alcohol. The present study demonstrates that larval zebrafish exert a response to the acute treatment of alcohol, which is genetically modifiable. Therefore, the larval zebrafish represent a tractable vertebrate model system for a large-scale genetic analysis of the biological effects of alcohol. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:647 / 654
页数:8
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