Increased 8-hydroxyguanine in DNA and its repair activity in hamster and rat lung after intratracheal instillation of crocidolite asbestos

被引：25

作者：

Yamaguchi, R ^{[1
]}

Hirano, T ^{[1
]}

Ootsuyama, Y ^{[1
]}

Asami, S ^{[1
]}

Tsurudome, Y ^{[1
]}

Fukada, S ^{[1
]}

Yamato, H ^{[1
]}

Tsuda, T ^{[1
]}

Tanaka, I ^{[1
]}

Kasai, H ^{[1
]}

机构：

[1] Univ Occupat & Environm Hlth, Inst Ind Ecol Sci, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1999年 / 90卷 / 05期

关键词：

8-hydroxyguanine; DNA repair; endonuclease nicking assay; asbestos; carcinogenicity;

D O I：

10.1111/j.1349-7006.1999.tb00776.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Asbestos and man-made-mineral fibers are known to increase one type of oxidative DNA damage, 8-hydroxyguanine (8-OH-Gua), in vitro. In this study, we analyzed the 8-OH-Gua level in DNA and its repair activity after a single intratracheal instillation of fibers (crocidolite or glass) or saline to Syrian hamsters or Wistar rats. The 8-OH-Gua level was measured with a high-performance liquid chromatography-electrochemical detector (HPLC-ECD) system. The 8-OH-Gua repair enzyme activity was determined with an endonuclease nicking assay using a P-32-labeled or fluorescently labeled 22mer DNA that contains 8-OH-Gua at a specific position. A significant increase in the 8-OH-Gua level in the lung DNA was observed 1 day after the exposure to crocidolite, as compared to the saline control. The repair activity was increased significantly at 7 days. On the other hand, after exposure to glass fibers, little or no increase of these carcinogenicity indicators was detected. These assays of 8-OH-Gua and its repair activity in short-term animal experiments will be useful for evaluating the carcinogenicity of fibers. This is the first report of the increase of 8-OH-Gua and its repair activity in the animal lung after the instillation of asbestos fibers.

引用

页码：505 / 509

页数：5

共 17 条

[1] OXIDATIVE DAMAGE ON DNA INDUCED BY ASBESTOS AND MAN-MADE FIBERS INVITRO [J].