Nonlinear laser imaging of skin lesions

被引：63

作者：

Cicchi, R. ^{[1
,2
]}

Sestini, S. ^{[3
]}

De Giorgi, V. ^{[3
]}

Massi, D. ^{[4
]}

Lotti, T. ^{[3
]}

Pavone, F. S. ^{[1
,2
]}

机构：

[1] Univ Florence, LENS, I-50019 Sesto Fiorentino, Italy

[2] Univ Florence, Dept Phys, I-50019 Sesto Fiorentino, Italy

[3] Univ Florence, Dept Dermatol, I-50121 Florence, Italy

[4] Univ Florence, Dept Human Pathol & Oncol, I-50134 Florence, Italy

来源：

JOURNAL OF BIOPHOTONICS | 2008年 / 1卷 / 01期

关键词：

multiphoton; fluorescence; second-harmonic generation; lifetime;

D O I：

10.1002/jbio.200710003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We investigated different kinds of human ex-vivo skin samples by combined two-photon intrinsic fluorescence (TPE), second-harmonic generation microscopy (SHG), fluorescence lifetime imaging microscopy (FLIM), and multispectral two-photon emission detection (MTPE). Morphological and spectroscopic differences were found between healthy and pathological skin samples, including tumors. In particular, we examined tissue samples from normal and pathological scar tissue (keloid), and skin tumors, including basal cell carcinoma (BCC), and malignant melanoma (MM). By using combined TPE-SHG microscopy we investigated morphological features of different skin regions. Further comparative analysis of healthy skin and neoplastic samples was performed using FLIM, and MTPE. Finally, we demonstrated the use of methyl-aminolevulinate as a contrast agent to increase the contrast in BCC border detection. The results obtained represent further support for in-vivo noninvasive imaging of diseased skin. [GRAPHICS] Elastin and collagen fibers inside human dermis imaged by combined two photon fluorescence (green) and second harmonic generation (blue) microscopy. (C) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：62 / 73

页数：12

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