Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC)

被引：23

作者：

Frasca, D

Barattini, P

Tirindelli, D

Guidi, L

Bartoloni, C

Errani, A

Costanzo, M

Tricerri, A

Pierelli, L

Doria, G

机构：

[1] ENEA, CR Casaccia, Immunol Lab, AMB PRO TOSS, I-00060 Rome, Italy

[2] Univ Cattolica Sacro Cuore, Inst Internal Med, Rome, Italy

[3] Univ Cattolica Sacro Cuore, Hematol Unit, Rome, Italy

[4] Univ Roma Tor Vergata, Dept Biol, I-00173 Rome, Italy

来源：

EXPERIMENTAL GERONTOLOGY | 1999年 / 34卷 / 05期

关键词：

ku protein; DNA repair; aging;

D O I：

10.1016/S0531-5565(99)00026-1

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

DNA binding of the ku protein was investigated in peripheral blood mononuclear cells (PBMC) from 24 subjects of different ages (20-89 years old) displaying age-related changes in DNA repair, mitotic responsiveness, and cytokine production. Ku is an heterodimeric protein composed of two subunits of 70 and 80 kDa, which is involved in the earliest steps of DNA damage recognition, DNA binding of ku 70/80 was found unchanged in normal PBMC from aging subjects but progressively declined in x-ray-irradiated PBMC from young to adult, and elderly subjects, This finding was concomitant with the age-related fall of DNA repair in the whole population. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：645 / 658

页数：14

共 35 条

[1] Saccharomyces cerevisiae Ku70 potentiates illegitimate DNA double-strand break repair and serves as a barrier to error-prone DNA repair pathways [J].