Nitrous oxide emissions from soil amended with untreated liquid manure (slurry), anaerobically digested slurry, or inorganic fertilizers (calcium ammonium nitrate or urea) were quantified in a field study revering two growth seasons of spring barley (Hordeum vulgare L.), In the first year incorporation before seeding was compared with trail hose application 5 wk after seeding, In the second year all fertilizers were applied before seeding, while soil moisture and initial NO(3)(-) availability was varied. Accumulated N(2)O losses between the time of fertilization and ca. 1 July represented 0.14 to 0.35%: of total N in 1996 and 0.34 to 0.64% in 1997, In both years the highest N(2)O emissions were observed with untreated slurry, whereas digested slurry and inorganic fertilizers Here at a similar level,Increasing the soil moisture content or NO(3)(-); availabiliiy had no significant effect on accumulated N(2)O losses. Although metabolizable C may thus have stimulated N, emissions via denitrification from untreated slurry, the largest contribution to N(2)O fluxes probably came from nitrification with all fertilizer types, Using the IPCC guidelines it was estimated that anaerobic digestion of slurry per se could potentially reduce N,O emissions from Danish agriculture hy 1.2 to 2.5%.