Human retinal pigment epithelial cells express scavenger receptors BI and BII

被引:71
作者
Duncan, KG
Bailey, KR
Kane, JP
Schwartz, DM
机构
[1] Univ Calif San Francisco, Dept Ophthalmol, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Cardiovasc Res Inst, San Francisco, CA 94143 USA
[3] Vet Affairs Med Ctr, San Francisco, CA 94121 USA
关键词
AMD; retinal pigment epithelium; scavenger receptor; lipid; photoreceptor outer segment;
D O I
10.1006/bbrc.2002.6756
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The retinal pigment epithelial (RPE) cells that underlie the retina ingest and metabolize thousands of lipid-rich photoreceptor outer segments (POS) every day. The scavenger receptor (SR) CD36 and integrin alphavbeta5 have been shown to participate in POS binding and internalization by RPE cells. The objective of the current study was to determine whether RPE cells express SRs other than CD36. Primary cultures of human RPE cells express both mRNA and protein for SR-BI and SR- BII. SR-BI and SR-BII mRNAs were detected by reverse transcription-polymerase chain reaction. SR-BI protein was detected by immune precipitation of [S-35]methionine-labeled crude cellular extracts. SR-BII was detected by Western blotting of immune precipitated crude cellular extracts. SR-BI and SR-BII proteins were also detected by immunofluorescence staining of RPE cells in culture. The results suggest that these SRs may play a role in POS lipid binding and uptake by RPE cells in the eye. (C) 2002 Elsevier Science (USA).
引用
收藏
页码:1017 / 1022
页数:6
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