Anti-PCNA autoantibodies preferentially recognize C-terminal of PCNA in patients with chronic hepatitis B virus infection

被引:20
作者
Hsu, TC
Tsay, GJ
Chen, TY
Liu, YC
Tzang, BS
机构
[1] Chung Shan Med Univ, Inst Immunol, Taichung 402, Taiwan
[2] Chung Shan Med Univ, Inst Biochem & Biotechnol, Taichung 402, Taiwan
[3] Chung Shan Med Univ Hosp, Dept Internal Med, Taichung, Taiwan
[4] Natl Tsing Hua Univ, Dept Life Sci, Hsinchu, Taiwan
关键词
proliferating cell nuclear antigen (PCNA); autoantibody; hepatitis B virus (HBV); hepatitis C virus (HCV); antigenic regions;
D O I
10.1111/j.1365-2249.2006.03046.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We previously reported anti-PCNA autoantibodies in sera from patients with chronic HBV and HCV infection. To analyse the antigenic regions on proliferating cell nuclear antigen (PCNA) that confer autoantibody binding in patients with chronic hepatitis B (HBV) and C (HCV) infection, eight constructs including one wild type PCNA, one mutant type Y114A_PCNA and six C- or N-terminal PCNA truncations were generated. Sera from 185 patients with systemic lupus erythematosus (SLE), 178 with chronic HBV and 163 with chronic HCV infection, and 68 healthy individuals were examined for the presentation of anti-PCNA antibodies by enzyme linked immunosorbent assay (ELISA). By ELISA, anti-PCNA positive sera from patients with SLE, chronic HBV and HCV infection preferentially recognized the wild type PCNA more than the mutant type Y114A_PCNA (P < 0.05). The inhibition of binding by purified full-length rPCNA proteins with anti-PCNA positive sera was shown to exceed 70%. The inhibition of binding by purified truncated rPCNA proteins with sera from patients with chronic HBV and HCV infection and SLE was shown to confer dominant binding in T-L2 and T-L3. Moreover, the higher frequency of inhibition by using T-L3 was found in patients with chronic HBV infection. These data indicate that anti-PCNA autoantibodies preferentially recognize C-terminal of PCNA in patients with chronic HBV infection and may also provide advanced understanding between viral infection and autoimmunity for further study.
引用
收藏
页码:110 / 116
页数:7
相关论文
共 35 条
[1]   Hepatitis B and C viruses serology in patients with SLE [J].
AbuShakra, M ;
ElSana, S ;
Margalith, M ;
Sikuler, E ;
Neumann, L ;
Buskila, D .
LUPUS, 1997, 6 (06) :543-544
[2]   The predictive value of fluctuations in IgM and IgG class anti-dsDNA antibodies for relapses in systemic lupus erythematosus. A prospective long term observation [J].
Bootsma, H ;
Spronk, PE ;
TerBorg, EJ ;
Hummel, EJ ;
deBoer, G ;
Limburg, PC ;
Kallenberg, CGM .
ANNALS OF THE RHEUMATIC DISEASES, 1997, 56 (11) :661-666
[3]  
BRAND SR, 1994, J IMMUNOL, V153, P3070
[4]   Hepatitis C virus, autoimmunity and rheumatic disease [J].
Buskila, D ;
Sikuler, E ;
Shoenfeld, Y .
LUPUS, 1997, 6 (09) :685-689
[5]  
COLACO CB, 1985, CLIN EXP IMMUNOL, V59, P449
[6]   CLINICAL-FEATURES OF PATIENTS WITH ANTIBODIES DIRECTED AGAINST PROLIFERATING CELL NUCLEAR ANTIGEN [J].
FRITZLER, MJ ;
MCCARTY, GA ;
RYAN, JP ;
KINSELLA, TD .
ARTHRITIS AND RHEUMATISM, 1983, 26 (02) :140-145
[7]   Autoantibodies and common viral illnesses [J].
Hansen, KE ;
Arnason, J ;
Bridges, AJ .
SEMINARS IN ARTHRITIS AND RHEUMATISM, 1998, 27 (05) :263-271
[8]   BASE-LINE SEROEPIDEMIOLOGY OF HEPATITIS-B VIRUS-INFECTION IN CHILDREN IN TAIPEI, 1984 - A STUDY JUST BEFORE MASS HEPATITIS-B VACCINATION PROGRAM IN TAIWAN [J].
HSU, HY ;
CHANG, MH ;
CHEN, DS ;
LEE, CY ;
SUNG, JL .
JOURNAL OF MEDICAL VIROLOGY, 1986, 18 (04) :301-307
[9]   INSIGHTS INTO NATIVE EPITOPES OF PROLIFERATING CELL NUCLEAR ANTIGEN USING RECOMBINANT-DNA PROTEIN PRODUCTS [J].
HUFF, JP ;
ROOS, G ;
PEEBLES, CL ;
HOUGHTEN, R ;
SULLIVAN, KF ;
TAN, EM .
JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (02) :419-429
[10]   RHEUMATIC MANIFESTATIONS OF HEPATITIS-B VIRUS-INFECTION [J].
INMAN, RD .
SEMINARS IN ARTHRITIS AND RHEUMATISM, 1982, 11 (04) :406-420