Organelle DNA and male fertility variation in Solanum spp. and interspecific somatic hybrids

被引:33
作者
Cardi, T
Bastia, T
Monti, L
Earle, ED
机构
[1] CNR, IMOF, Res Inst Vegetable & Ornamental Plant Breeding, I-80055 Portici, Italy
[2] Cornell Univ, Dept Plant Breeding, Ithaca, NY 14853 USA
关键词
interspecific hybridization; Solanum; male fertility; chloroplasts; mitochondria;
D O I
10.1007/s001220051301
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Novel and potentially useful genetic variation in cytoplasmic genomes can be induced by interspecific somatic hybridization in plants. To evaluate such variability and correlate it with nuclear-cytoplasmic interactions leading to male sterility in Solanum spp., we examined progeny of male-sterile and male-fertile somatic hybrids between Solanum tuberosum (tbr), the common potato, and S. commersonii (cmm), a wild species showing sexual incongruity with tbr, for fertility and organelle DNA composition. Uniform male-fertile and male-sterile progenies were obtained by selfing the male-fertile hybrid and crossing the male-sterile ones, indicating maternal inheritance of the fertility phenotype. The two fusion partners were only slightly differentiated in the plastidial genome. MtDNA polymorphism between the species was greater, although its extent varied with the genomic region investigated. All somatic hybrids had non-parental organelle genomes, with reasserted organelles and/or rearranged mitochondria (i.e., cmm-specific bands for some regions and tbr-specific bands for others). Mitochondria reasserted independently from chloroplasts. Most hybrids showed the cmm cpDNA hybridization pattern, indicating non-random transmission of chloroplasts. Most male-sterile hybrids showed preferential inheritance of tbr mtDNA fragments. The male-fertile somatic hybrid clone had predominantly cmm mtDNA fragments. This result suggests that a tbr-derived region involved in nuclear-cytoplasmic incompatibility and male sterility has been lost by rearrangement; however, no clear correlation between a specific mitochondrial region and male sterility has been found so far.
引用
收藏
页码:819 / 828
页数:10
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