Immunocytochemical localization of nitric oxide synthase in the mammalian retina

被引:46
作者
Kim, IB
Lee, EJ
Kim, KY
Ju, WK
Oh, SJ
Joo, CK
Chun, MH
机构
[1] Catholic Univ Korea, Coll Med, Dept Anat, Seoul 137701, South Korea
[2] Catholic Univ Korea, Coll Med, Dept Ophthalmol, Seoul 137701, South Korea
关键词
nitric oxide synthase; retina; rat; mouse; guinea pig; rabbit; cat;
D O I
10.1016/S0304-3940(99)00363-8
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The localization of nitric oxide synthase (NOS) was investigated by immunocytochemistry and immunoblotting using an antiserum against neuronal NOS in the rat, mouse, guinea pig, rabbit and cat retinae. Western blot analysis of retinal tissue extracts showed that the NOS-immunoreactive band of 155 kDa was present in all species. In the rat, mouse, guinea pig and rabbit retinae, two types of amacrine cells and a class of displaced amacrine cells were consistently NOS-labeled. In the cat retina, unlike other mammals, one type of amacrine cells and two types of displaced amacrine cells showed NOS immunoreactivity. NOS immunoreactivity was further found in some bipolar cells of the rat and guinea pig, some interplexiform cells of the mouse, some photoreceptor cells of the rabbit and same Muller cells of the cat. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:193 / 196
页数:4
相关论文
共 19 条
  • [1] RETINAL GANGLION-CELLS EXPRESS A CGMP-GATED CATION CONDUCTANCE ACTIVATABLE BY NITRIC-OXIDE DONORS
    AHMAD, I
    LEINDERSZUFALL, T
    KOCSIS, JD
    SHEPHERD, GM
    ZUFALL, F
    BARNSTABLE, CJ
    [J]. NEURON, 1994, 12 (01) : 155 - 165
  • [2] NO news from insect brains
    Bicker, G
    [J]. TRENDS IN NEUROSCIENCES, 1998, 21 (08) : 349 - 355
  • [3] Bredt DS, 1992, NEURON, V8, P3
  • [4] LOCALIZATION OF NADPH-DIAPHORASE/NITRIC OXIDE SYNTHASE IN THE RAT RETINA - AN ELECTRON-MICROSCOPIC STUDY
    DARIUS, S
    WOLF, G
    HUANG, PL
    FISHMAN, MC
    [J]. BRAIN RESEARCH, 1995, 690 (02) : 231 - 235
  • [5] NITRIC-OXIDE SYNTHASE AND NEURONAL NADPH DIAPHORASE ARE IDENTICAL IN BRAIN AND PERIPHERAL-TISSUES
    DAWSON, TM
    BREDT, DS
    FOTUHI, M
    HWANG, PM
    SNYDER, SH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) : 7797 - 7801
  • [6] MONO ADP-RIBOSYLATION OF TRANSDUCIN CATALYZED BY ROD OUTER SEGMENT EXTRACT
    EHRETHILBERER, S
    NULLANS, G
    AUNIS, D
    VIRMAUX, N
    [J]. FEBS LETTERS, 1992, 309 (03): : 394 - 398
  • [7] Elphick M R, 1997, Methods Mol Biol, V72, P153
  • [8] Nitric oxide: A review of its role in retinal function and disease
    Goldstein, IM
    Ostwald, P
    Roth, S
    [J]. VISION RESEARCH, 1996, 36 (18) : 2979 - 2994
  • [9] Nitric oxide modulates cGMP levels in neurons of the inner and outer retina in opposite ways
    Gotzes, S
    de Vente, J
    Müller, F
    [J]. VISUAL NEUROSCIENCE, 1998, 15 (05) : 945 - 955
  • [10] NADPH-DIAPHORASE EXPRESSION IN NEURONS AND GLIA OF THE NORMAL ADULT-RAT RETINA
    HUXLIN, KR
    [J]. BRAIN RESEARCH, 1995, 692 (1-2) : 195 - 206